摘要
目的研究左向右分流先心病肺动脉高压(肺高压)患儿肺组织内皮素-1(ET-1)与肺组织Ⅰ、Ⅲ型胶原合成的关系,探讨左向右分流先心病患儿肺高压形成的机理。方法选择左向右分流先心病患儿18例,根据肺动脉收缩压分为肺高压组(n=10),非肺高压组(n=8)。所有患儿在外科手术体外循环开始前,取右肺中叶组织(1cm×1cm×1cm),放射免疫法测定患儿血浆及肺组织ET-1含量;RT-PCR法检测肺组织Ⅰ、Ⅲ型胶原mRNA表达。结果先心病肺高压患儿血浆及肺组织ET-1含量明显高于肺动脉压正常者(P<0.01)。肺高压患儿肺组织Ⅰ、Ⅲ型前胶原mRNA的表达与肺动脉压正常者相比明显增加(P<0.05)。结论ET-1可能通过刺激肺动脉平滑肌细胞胶原合成增加参与肺高压的形成。
Objective To explore the pathogenesis of pulmonary hypertension in children with left to right shunt congenital heart disease （CHD） and the relationship of endothelin-1 （ET-1） and typeⅠ and type Ⅲ collagen synthesis in pulmonary tissue. Methods 18 children with CHD were enrolled in this study, and the right middle lung tissue （1cm×1cm×1cm） were dissected before extrasystolic circulation. The plasma and pulmonary tissue ET-1 were detected with radioimmunoassay, and the expression of the mRNA of type Ⅰ and type Ⅲ procollagen in lung tissue was measured with RT-PCR. Results The plasma and pulmonary tissue ET-1 levels as well as the expression levels of the mRNA of type Ⅰ and type Ⅲ procollagen in lung tissue were significantly higher in pulmonary hypertension group than those in non-pulmonary hypertension group （P〈0.05）. Conclusions ET-1 can increase the collagen level of lung tissue that maybe involved in the pathogenesis of pulmonary hypertension through stimulating the collagen synthesis of pulmonary arterial smooth muscle cells.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2006年第5期374-376,共3页
Journal of Clinical Pediatrics
关键词
内皮素-1
胶原合成
肺动脉高血压
先天性心脏病
endothelin-1
collagen synthesis
pulmonary hypertension
congenital heart disease
