摘要
目的建立左向右分流所致肺动脉高压大鼠模型,了解高肺血流量对肺血管胶原代谢的影响。方法对大鼠行腹主动脉下腔静脉分流术。术后11周以右心导管法测定肺动脉平均压(PAMP),采用免疫组织化学法检测大鼠肺动脉Ⅰ、Ⅲ型胶原蛋白的表达。结果分流11周后大鼠肺循环与体循环血流量之比分别为3.3∶1.0,为大量左向右分流。PAMP较对照组明显升高(P<0.01)。分流组大鼠肺中、小型肺动脉中Ⅰ、Ⅲ型胶原蛋白表达与对照组比较明显增加。结论高肺血流量可导致肺动脉高压,并促进肺动脉高压大鼠细胞外基质/胶原的堆积。
Objective To establish a rat model of pulmonary hypertension induced by left - to- right shunt and explore the influence of high pulmonary blood flow on pulmonary viscular collagen remodeling. Methods Abdominal aorta and inferior vena cava shunting was produced in rats. Pulmonary artery meanpressure （PAMP） of each rat was measured by using a right cardiac catheterization. Pulmonary artery collagen Ⅰ and Ⅲ were detected using immunohistochemisty. Results After 11 weeks of shunting the Qp/Qs was 3.3：1.0, indicating a large shunt. Pulmonary artery mean pressure was increased as compared with controls[ （23.0 ± 0.9） mm Hg vs （ 15.7 ± 1.1 ）mm Hg, P〈0.01] and pldmonary hypertension developed. Pulmonary artery collagen Ⅰ and Ⅲ expressions were markedly strengthened compared with control rats. Conclusion Increased- pulmonary blood flow can induce pulmonary hypertension and augment the pulmonary collagen remodeling.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2005年第12期1183-1184,1197,共3页
Journal of Applied Clinical Pediatrics
关键词
肺动脉高压
左向右分流
大鼠
胶原
pulmonary hypertension
left - to - right shunt
rat
collagen
