摘要
目的研究灵芝对鹅膏毒菌中毒兔的解救及心肌的保护作用。方法新西兰兔随机分成正常对照组、模型组、灵芝治疗组Ⅰ(20 g.kg-1)、灵芝治疗组Ⅱ(60 g.kg-1),连续灌胃5 d,取血检查血清天门冬氨酸氨基转移酶(AST)、血清乳酸脱氢酶(LDH)、α-羟丁酸(α-HBDH)、肌酸激酶(CK)、肌酸激酶同功酶(CK-MB),处死动物后进行病理组织学观察。结果模型组动物在给予鹅膏毒菌提取液5 d后存活率为60%,其他组为100%;模型组兔在鹅膏毒菌提取液灌胃后2 d,心肌各项酶活性AST、LDH、α-HBDH、CK、CK-MB显著高于正常对照组,第5 d进一步增高,2个剂量的灵芝煎剂能降低各项酶活性,呈剂量依赖倾向。结论灵芝煎剂能明显提高鹅膏毒菌中毒兔的存活率,灵芝减轻鹅膏毒菌所致心肌损伤是其解毒机制之一。
OBJECTIVE To investigate the protective effects of Ganoderma lucidum on heart damage caused by am anita poisoning. METHODS Twenty healthy New Zealand white rabbits were randomly divided into 4 groups., group A （control）, group B （organic phosphorus poison, namely amanita, was given through gastric canal at 100 mg · kg^-1 ）, group C （ganoderma lucidum was given through gastric canal at 20 g · kg^-1 for 5 days）, and group D （gano derma lucidum 60 g · kg ^-1 for 5 days）. Plasma contents of AST, ALT, LDH, a-HBDH, CK, and CK-MB were measured. Pathological changes in the heart was investigated and the survival rate of the rabbits was calculated. RE- SULTS The survival rate of group B was 60.0% （4/6）, that for group C and D was 100% （6/6）. The plasma con tents of AST, ALT, LDH, α-HBDH, CK, and CK-MB were significantly increased in group B compared with those in group A, C and D. The levels of AST, ALT, LDH, α-HBDH, CK, and CK MB in group C and D were significantly lower than those of group B and were not significantly lower than those of group A. Pathological changes of the heart were more obvious in group B than those of group C and D. CONCLUSIONS Ganoderma lucidum has protective effects on heart injury induced by amanita poisoning.
出处
《中南药学》
CAS
2006年第3期171-174,共4页
Central South Pharmacy
基金
湖南省科技厅国际合作处资助项目
编号:05WK3018
关键词
鹅膏毒菌
灵芝
心肌酶
组织形态学
amanita
ganoderma lucidum
myocardial zymogram
morphology
