摘要
目的:研究褪黑素对海马CA3区长时程增强(LTP)诱导阶段的影响及可能的机制。方法:用细胞外电生理记录方法,通过向大鼠海马CA3区分别微量注射褪黑素、Tacrine(胆碱酯酶抑制剂)和DNQX(非NMDA受体的竞争性拮抗剂),以海马CA3区群体兴奋性突触后电位(fEPSP)斜率的改变为指标,观察PP-CA3通路的LTP变化。结果:①0.2μg/μl、1μg/μl和5μg/μl的褪黑素对CA3区的诱发电位和LTP均有抑制作用,随着剂量加大,抑制作用加强。②褪黑素能抑制Tacrine对LTP诱导的易化作用。③DNQX能部分阻断褪黑素对LTP诱导的抑制作用。结论:褪黑素对LTP诱导的抑制作用可能与胆碱能系统和非NMDA受体有关。
Aim： To study the effect of melatonin on the induction of LTP in CA3 area of hippocampus and to investigated its possible mechanisms. Methods： Melatonin and other drugs （Tacrine or DNQX） were microinjected into the CA3 area. By using extracellular electrophysiological recordings to observe the changes of the slope of fEPSP in the CA3 area. Results： ① Evoked potential and the induction of LTP were depressed by different concentration of melatonin（0.2 μg/μl, 1 μg/μl and 5μg/μl）. As the melatonin concentration increased, the induction of LTP was blocked more obviously. ②Melatonin could attenuate the excitation effect of Tacrine （inhibitor of ACHE） on LTP. ③ Inhibition of the melatonin-induced on LTP attenuated by DNQX. Conclusion： The application of melatonin in rats inhibits the induction of LTP in the hippocampal CA3 area. The action of melatonin on the induction of LTP may be through the modulation of not only non-NMDA receptors but also cholinergic system.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2006年第2期195-199,共5页
Chinese Journal of Applied Physiology
