摘要
目的:通过观察慢性温和应激对大鼠行为及5-羟色胺、色氨酸、应激激素的影响,进一步阐明应激致抑郁发生的机制。方法:利用慢性不可预计温和应激(CUMS)结合行为学指标建立大鼠抑郁动物模型;高效液相检测血浆和脑5-HT、儿茶酚胺;放免方法检测血浆皮质醇换算成皮质酮含量;氨基酸分析仪检测血清色氨酸的含量。结果:①CUMS诱导8周大鼠糖水偏爱性与对照组相比明显减低(P<0.05);体重和旷场实验评分下降(P<0.05)。②CUMS诱导8周大鼠大脑和血浆5-HT含量显著下降(P<0.05);而血清色氨酸含量与对照组相比则明显升高(P<0.05)。③CUMS诱导8周大鼠血浆去甲肾上腺素和肾上腺素含量与对照组相比明显升高(P<0.05);血浆皮质酮含量与对照组相比统计学上无显著性差异(P>0.05)。结论:利用CUMS建立的抑郁模型大鼠存在着神经内分泌紊乱,CUMS大鼠抑郁样行为的发生可能与血浆去甲肾上腺素和肾上腺素升高对色氨酸代谢的影响有关。
Aim：To elucidate the mechanism of depression induced by chronic unpredictable mild stress（ CUMS）, the effects of CUMS on serotonin（5-HT）, trytophan, stress hormones and behaviour were investigated in rats. Methods： Depression was inducedby for 8 weeks CUMS and confirmed by behavioral tests, the brain and plasma levels of monoamine neurotransmitters were analyzed by HPLC-ECD techniques, the content of plasma corticosterone was evaluated by I^125cortisol radioactivity immunoassay and the serum tryptophan content was measured by HTTACHI L-8800 amino acid analyzer. Results： ①Rats exposed to a series of mild, unpredictable stressors for 8weeks displayed the decreased body weight, reduced scores of open-field test and preference of sucrose solution （P〈0.05）. ② Plasma and brain 5-HT contents in rats after exposure to CUMS 8 weeks decreased significantly（P〈0.05）. While serum tryptophan content increased at the same time（P 〈 0.05）. ③Plasma norepinephrine and epinephrine in rats were increased after CUMS 8 weeks, but there was no difference between control and CUMS group in plasma corticosterone. Conclusion： The beha vioral changes induced by CUMS for 8 weeks are similar to the features of human depression, which may be related to the disturbances of tryptophan metablism induced by increased norepinephrine and epinephrine in CUMS rat.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2006年第2期169-172,共4页
Chinese Journal of Applied Physiology
