摘要
目的检测乙型肝炎所致的终末期肝病患者肝移植前、后血清乙型肝炎病毒(HBV)DNA的含量,探讨HBV再感染的检测方法。方法采用荧光定量聚合酶链反应(FQPCR)技术检测275例乙型肝炎所致终末期肝病患者肝移植前以及肝移植后1周、1个月、3个月的血清HBVDNA含量,同时采用酶联免疫吸附试验(ELISA法)检测HBV标志物。结果275例血清标本中,术前乙型肝炎表面抗原(HBsAg)、乙型肝炎核心抗体(抗-HBc)均为阳性;HBsAg、乙型肝炎e抗原(HBeAg)及抗-HBc阳性者的血清HBVDNA均为阳性;HBsAg、乙型肝炎e抗体(抗-HBe)及抗-HBc阳性者73.8%的血清HBVDNA阳性;HBsAg及抗-HBc阳性者67.6%的血清HBVDNA阳性。术后1周患者的HBsAg及HBVDNA均为阴性。术后1个月,69例HBsAg转为阳性,其中48例(69.6%)HBVDNA为阳性。术后3个月,137例HBsAg为阳性,其中104例(75.9%)HBVDNA为阳性。结论乙型肝炎所致的终末期肝病患者肝移植后HBV的再感染率较高,应用FQPCR检测HBVDNA含量能更准确地反映体内HBV的复制情况,可与HBV标志物检测互补。
Objective To determine the nucleic acid content of hepatitis B virus with different clinical types of hepatits B before,after liver transplantation patients and explore the approach for detection of hepatitis B. Methods The HBV DNA content in before the liver transplantation, 7 day, 30 day, 90 day after the liver transplantation was detected with fluorescent quantitative PCR and HBV M with ELISA in 275 liver transplantation patients. Results HBsAg, HBcAb was positive of 275 patients; HBV DNA was positive in all HBsAg（ ＋ ）/HBeAg（ ＋ ）/Hl3cAb（ ＋ ） specimens;79（73. 8%） out of the 107 HBsAg（ ＋ ）/HBeAb（ ＋ ）/Hl3cAb（ ＋ ） specimens; 48（67. 6 %）out of the 71 HBsAg （ ＋ ）/HBcAb（ ＋ ） specimens in before the liver transplantation. HBV DNA was negative in out of the 275 HBsAg（ - ） in 7 day after the liver transplantation; 48（69. 6%） out of the 69 HBsAg（ ＋ ） in 30 day after the liver transplantation; 104（75.9 %） out of the 137 HBsAg（ ＋ ） in 90 day after the liver transplantation. Conclusions The recurrent hepatitis B were higher in the different clinical types of hepatitis B after liver transplantation,Detection of HBV DNA with fluorescent quantitative PCR can accurately reflect the state of HBV infection and HBV replication and HBV DNA was complementarity with HBV M, it can be used to guide clinical diagnosis with recurrent hepatitis B.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2006年第5期283-284,共2页
Chinese Journal of Organ Transplantation
关键词
肝移植
肝炎病毒
乙型
感染
Liver transplantation
Hepatitis B virus
Infection
