摘要
目的:检测我国新生儿脐带血T细胞亚群的变化。方法:使用全自动血液分析仪进行血常规检测;荧光素标记的抗CD4／抗CD8／抗CD3和抗CD4／抗CD25／抗CD3单克隆抗体对血液细胞表面分子进行免疫荧光染色, 用流式细胞术进行各细胞亚群计数和分析。结果:新生儿脐带血中红细胞(RBC)计数、血红蛋白(Hb)含量、白细胞(WBC)计数、淋巴细胞百分比与成人外周血有显著性差异。新生儿脐带血组中CD3+T细胞、CD3+CD4+T细胞、CD3+CD8+T细胞、CD4+CD8+T细胞、CD4+CD25high调节性T细胞、CD4+CD25intT细胞、CD4+CD25-T细胞绝对计数及其在各自细胞亚群中的百分比均存在显著性差异。结论:中国正常新生儿脐带血与成人外周血的 RBC、Hb、WBC及T细胞亚群检测结果存在显著性差异,这与新生儿免疫机制发育不完全是相符合的。
Objective： To study the situation of T cell subpopulations in human umbilical cord and adult peripheral blood. Methods： Full blood differential counts were determined by blood routine examination. Stain the blood cells with fluorescent labeled anti-CD4/ anti-CD25/ anti-CD3 and anti-CD4/ anti- CD8/anti-CD3 monoclonal antibody. Samples were run and analyzed for three-color immunofluorescence by flow cytometry. Results ： The contents of erythrocytes ,leucocytes ,haematoglobin and the percentage of lympholeukocyte in umbilical cord blood were markedly differed from those in adult peripheral blood. The absolute count and the percentage of CD3 ^＋ T cells, CD3 ^＋ CD4 ^＋ T cells, CD3 ^＋ CD8 ^＋ T cells, CD4 ^＋ CD8^＋T cells,CD4^＋ CD25^high regulatory T cells,CD4^＋ CD25^int T cells and CD4^＋ CD25^- T cells were markedly differed from those in adult peripheral blood. Conclusion： The full blood differential counts and the T cell subpopulations in cord were markedly differed from those in adult peripheral blood. This result is probably corresponding with the immune state of the neonates.
出处
《医学研究生学报》
CAS
2006年第5期399-401,404,共4页
Journal of Medical Postgraduates
基金
教育部归国人员启动基金资助项目(批准号:2002-HG003)第四军医大学人才基金资助项目(2003年度)第四军医大学唐都医院苗子人才基金资助项目(2001年度)
关键词
T细胞亚群
新生儿脐带血
成人外周血
流式细胞术
T cell subpopulations
Human umbilical cord blood
Adult peripheral blood
Flow cytometry
