摘要
Polyamines are implicated in regulating various developmental processes in plants, but their exact roles and how they govern these processes still remain elusive. We report here an Arabidopsis bushy and dwarf mutant, bud2, which results from the complete deletion of one member of the small gene family that encodes S-adenosylmethionine decarboxylases （SAMDCs） necessary for the formation of the indispensable intermediate in the polyamine biosynthetic pathway. The bud2 plant has enlarged vascular systems in inflorescences, roots, and petioles, and an altered homeostasis ofpolyamines. The double mutant of bud2 and samdcl, a knockdown mutant of another SAMDC member, is embryo lethal, demonstrating that SAMDCs are essential for plant embryogenesis. Our results suggest that polyamines are required for the normal growth and development of higher plants.
基金
We thank Dr Senya Matsufuji(Jikei University,Japan)for providing the yeast spe2 strain,Dr Guixian Xia(Institute of Microbiology,Chinese Academy of Sciences,China)for yeast expression vector pREP5N and ABRC stock center for BAC clones(F17K4 and T16G12)and T-DNA insertion lines(Salk_007279 and Salk_020185).This work was supported by grants from National Natural Science Foundation of China(30330040)
the Ministry of Sciences and Technology of China(J02-A-001).
