摘要
【目的】观察补肾益气活血方骨炎定对血管内皮细胞增殖的影响。【方法】将新西兰兔随机分为空白组和骨炎定低、中、高剂量组（剂量分别为2，5、5、10g/kg）；以骨炎定高剂量灌胃给药，连续7d，于最后1次给药后0，5、l、1．5、2、2，5、3、3．5、4h分别取血，分离含药血清，作用于人脐静脉内皮细胞，以四甲基偶氮唑盐（MTT）法检测不同时相的骨炎定含药血清对血管内皮细胞的增殖作用；并取给药后2h含药血清，部分灭活，比较灭活血清与不灭活血清对血管内皮细胞增殖的影响，以及检测不灭活含药血清不同作用时间（12、24、36h）对内皮细胞增殖的影响；以骨炎定低、中、高剂量灌胃给药，连续7d，取最后1次给药后2h含药血清，观察不同剂量骨炎定对血管内皮细胞增殖的影响。【结果】高剂量骨炎定给药后0．5～4h制备的含药血清对血管内皮细胞增殖均有显著促进作用（均P〈0．01），其中以给药后2h者作用最强；灭活与不灭活血清均对血管内皮细胞增殖具有促进作用（P〈0．01），且后者作用强于前者（P〈0．05）；不灭活含药血清作用12、24、36h后均对血管内皮细胞增殖具有促进作用（P〈0．01），且呈时效关系；低、中、高剂量骨炎定含药血清均对血管内皮细胞具有促进作用（P〈0．01），并呈量效关系。
[ Objective] To observe the effect of Guyanding （GYD） decoction, a prescription with the actions of tonifying kidney, strengthening Qi and activating blood on proliferation of vascular endothelial cells （VEC）. [ Methods] New Zealand rabbits were randomized into blank control groups and GYD decoction groups in the dosages of 2.5, 5 and 10g/kg respectively. GYD in the dosage of 10g/kg was given to rats for 7 successive days, and blood sampling was collected 0.5, 1, 1.5, 2, 2.5, 3, 3.5 and 4 hours after the last administration to prepare serum containing GYD. Then human umbilical vein endothelial cells were cultured with serum containing GYD, and the effects of GYD- containing serum at different time points on the proliferation of VEC were observed by methylthiazolyhetrazolium （MTY） assay. GYD-containing serum collected 2h after the last administration was partially inactivated and its effect on VEC proliferation was compared with that of non-inactivated GYD-containing serum. Meanwhile, the effect of non-inactivated GYD-containing serum on VEC proliferation was observed 12, 24 and 36 hours after the culturing. GYD in three different dosages was given to rats for 7 successive days, and serum containing GYD collected 2h after the last administration was prepared to observe the effect of GYD in different doses on VEC proliferation. [ Results ] Serum containing high-dose GYD collected 0.5 - 4h after the administration had obvious effect in promoting VEC proliferation （ P 〈 0.01 ）, and the effect of the serum collected 2h after administration was the strongest. Both inactivated serum and non-inactivated serum promoted VEC proliferation （ P 〈 0.01 ）, the effect of the latter being stronger than that of the former （P 〈 0.05）. Non-inactivated serum also promoted VEC proliferation 12, 24 and 36 hours after the culturing （P 〈 0.01 ）, and the effect was in a time-effect manner. Serum containing different doses of GYD promoted VEC proliferation in a dose-dependent manner （ P 〈 0.01 ）. [
出处
《广州中医药大学学报》
CAS
2006年第3期245-248,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
