摘要
采用冷浸乙醇提取法对兰州人工引种栽培的6年生乌拉尔甘草Glycyrrhiza uralensis根中的总黄酮和总皂甙类化合物进行了提取分离,并且采用重量法和3种紫外-可见分光光度法对乌拉尔甘草根中总黄酮含量进行了测定和比较分析。结果表明,3种分光光度测定方法中,黄酮含量以直接测定法为最高,硝酸铝显色法次之,氯化铝显色法最低,直接测定法测定样品中的黄酮含量因不受显色剂干扰,测定结果相对更为准确可靠。人工栽培乌拉尔甘草相对野生甘草有效成分的含量较低,但仍可作为野生药用甘草的替代品。
The methods of extraction and separation of total triterpene and flavonoids constituents from the roots of cultivated Glycyrrhiza uralensis in Lanzhou were studied. The contents of total triterpene and flavonoids were detected through the weight method. Meanwhile, the concentration of the total flavonoids in the extraction was also analyzed by the ultraviolet-visible spectrophotometry with three different methods. The results showed that,of the three methods, the concentration of total flavonoids was the highest by using the direct mensuration method, and the coloration method by Al（NO3）3 and AlCl3 took the second and the third place. The result of total flavonoids analyzed by the direct mensuration method was more accurate and reliable relatively. Though the contents of the effective components from the roots of cultivated G. uralensis are lower than that of wild G. uralensis, it can act as the replacer of wild officinal liquorice.
出处
《草业科学》
CAS
CSCD
2006年第5期48-52,共5页
Pratacultural Science
基金
甘肃省自然科学基金项目(ZS001-A25-011-Z)
关键词
乌拉尔甘草
总黄酮
皂甙
提取
分析
Glycyrrhiza uralensis
flavonoids
triterpene
extraction
analysis
