摘要
目的研究新疆紫草的乙醇提取物(AEE)抗大鼠肝窦阻塞综合征(SOS)作用。方法采用野百合碱(Mct)灌胃方法制备SOS大鼠模型。将50只SD大鼠随机分为5组,每组10只:A组(正常对照组)、B组(Mct160模型组)、C组(Mct160-AEE给药组)、D组(Mct100模型组)和E组(Mct100-AEE给药组)。在实验第0天,B,C组和D,E组分别予Mct160mg·kg^(-1)和Mct100mg·kg^(-1)灌胃,得到不同程度的SOS大鼠模型。其中C,E2个AEE给药组从应用Mct前2天开始灌胃AEE1g·kg^(-1)·d^(-1),持续给药7d后处死动物,测定腹水量、肝/体比、白细胞计数(WBC)、红细胞压积(HCT)、血清总胆红素水平、血清TNF-α水平,并进行肝脏病理检查,以了解AEE抗SOS的效果。结果C组与B组相比,腹水生成明显减少、血胆红素降低、HCT增高,且TNF-α水平有下降趋势;E组与D组相比,肝脏病理损害明显减轻,与正常对照组相近。结论口服新疆紫草的乙醇提取物可以在一定程度上预防大鼠的SOS。
OBJECTIVE To explore the effect of the Arnebia euchroma mot alcoholic extracts （AEE） on sinusoidal obstruction syndrome （SOS） in vivo .METHODS SOS rat model was established by the administration of monocrotaline （Mct）. Fifty SD rats were divided equally into 5 groups. Ten SD rats in group A were worked as normal control. Ten SD rats in group B were treated with Mct 160 mg·kg^-1 only;Ten rats in group C received AEE 1 g·kg^-1·d^-1 before being treated with Mct 160 mg·kg^-1 ; Ten SD rats in group D were treated with Mct Ten mg·kg^-1 only;Ten SD rats in group E received AEE 1 g·kg^-1·d^-1 before being treated with Mct 100 mg·kg^-1. The rats in group C and group E were given AEE for 7 days from two days before the application of Mct. At the end of the experiment, all rats were sacrificed. Their ascites, the ratio of liver/body weight, serum total bilimbin （TBil）, white blood cell count, hematocrit （HCT） and serum TNF-α in each group were measured, and the liver tissues were taken for histological examination. RESULTS The amount of ascites and the levels of TBil were significantly decreased and the values of HCT were increased in group C compared with that in group B; and significantly reduced liver injury was seen in group E compared with that in group D. CONCLUSION SOS in rat model was prevented partially by the oral application of AEE. It is suggested that Arnebia euchroma has potential to be used in the prophylaxis of SOS.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2005年第21期1626-1629,共4页
Chinese Pharmaceutical Journal
关键词
新疆紫草
肝窦阻塞综合征/肝静脉闭塞病
药物疗法
野百合碱
Arnebia euchrorna
sinusoidal obstruction syndrome/hepatic veno-occlusive disease
drug therapy
monocmtaline
