摘要
目的 探讨加替沙星在家兔体内的药动学特性。方法 采用反相高效液相色谱内标法，色谱柱：ORBAX Extend-C18柱，流动相：0．01mol·L^-1磷酸二氢钾缓冲液-乙腈（18：5）．流速1．0mL·min^-1，室温25℃。紫外检测波长为292nm．内标物为诺氟沙星。分析耳缘静脉推注加替沙星（10和20mg·kg^-1）后家兔体内的血药浓度，用药物和统计软件（DAS ver1．0）进行房室模型拟合并计算药动学参数。结果 加替沙星在0．025～4μg·mL^-1内线性关系良好（r=0．9973），在家兔体内药-时曲线符合一室模型。T1／2为1．25h。结论 本方法操作简便，结果准确．能满足加替沙星药动学分析。
OBJECTIVE To study the pharmacokinetics of gatifloxacin in rabbit . METHODS The plasma concentrations of gatifloxacin were determined by RP-HPLC method. The detection wavelength was 292nm,and norfloxacin was used as the internal standard. Data were disposed with DAS（Ver1. 0 software. RESULTS The concentration time curve of gatifloxacin was fitted to on-compartment model. T1/2 was 1.25h. CONCLUSION The study can provide pharmacokinetics parameters for rabbit. This method is simple and sensitive.
出处
《海峡药学》
2006年第2期142-144,共3页
Strait Pharmaceutical Journal
