摘要
研究了 2 ,4 二氯苯基荧光酮 (DCIPF)与Fe(Ⅲ )体系的荧光特性及反应条件。试验了多种表面活性剂和共存离子的影响。拟定了荧光熄灭法测定微量Fe的方法。该方法在pH 5 2～ 5 9NaAc HAc缓冲介质中 ,Fe(Ⅲ )与DCIPF反应生成红色配合物 ,使DCIPF溶液的荧光明显熄灭 ,其激发波长λex =2 82nm ,发射波长λem =5 6 0nm ,Fe的浓度在 4～ 2 4ng·mL- 1 范围内符合比耳定律 ,相关系数为 0 9996。对于 16ng·mL- 1 的Fe测定 11次的相对标准偏差为 4 0 %。本方法用于直接测量中草药、茶叶标样中微量铁 ,结果较好。
A new spectrofluorimetric method for the determination of trace amount of Fe(Ⅲ) has been developed. This method is based on the fluorescence quenching of 2,4-dichro-phenylfluorone due to the formation of complex Fe(Ⅲ)-DCIPF. In pH 5.2-5.9 buffer solution, Fe(Ⅲ) and DCIPF react on each other to form a red complex. Its composition was established by method of continuous variations and molar-ratio as Fe(Ⅲ)∶DCIPF =1∶4, the excitation and emission wavelengths were found to be 282 and 560 nm, respectively. There is a linear relationship in the range 4-24 ng·mL -1 for Fe(Ⅲ). This method is simple and rapid, and has been applied to the determination of trace iron in Chinese herbal medicine and tea samples with satisfactory results.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第7期848-850,共3页
Spectroscopy and Spectral Analysis
基金
内蒙古自治区自然科学基金项目 (2 0 0 2 0 80 2 0 1 1 4 )资助
