摘要
目的测定正常大鼠、地塞米松处理大鼠以及小鼠感染弓形虫前后的血清一氧化氮水平,探讨弓形虫感染对其血清一氧化氮水平的影响。方法将36只雌性SD大鼠分为6组,每组6只。Ⅰ组:正常对照组;Ⅱ组:弓形虫感染组;Ⅲ组:地塞米松2mg/kg体重,隔天皮下注射2周后停止注射;Ⅳ组:地塞米松2mg/kg体重,隔天皮下注射2周后感染弓形虫,并停止注射地塞米松;Ⅴ组:实验期间,地塞米松2mg/kg体重,隔天皮下注射;Ⅵ组:地塞米松2mg/kg体重,隔天皮下注射2周后感染弓形虫,继续注射地塞米松。每周采集实验大鼠血液并分离血清。第6周时处死大鼠,收集血清,将弓形虫感染鼠的脑组织匀浆转种小鼠,并以脑组织为模板PCR扩增弓形虫SAG1基因。雌性昆明小鼠36只,于感染弓形虫后0,1,2,3,4,5d时每天处死6只,收集血清。硝酸根还原酶法测定大鼠、小鼠血清一氧化氮浓度。结果正常组大鼠6周平均一氧化氮浓度为(29.30±2.77)μmol/L;地塞米松注射2周后,Ⅲ组、Ⅳ组、Ⅴ组、Ⅵ组大鼠血清一氧化氮水平分别为(13.24±2.30)μmol/L、(13.33±2.41)μmol/L、(14.98±1.71)μmol/L、(14.55±2.99)μmol/L;Ⅱ组大鼠感染弓形虫后,血清一氧化氮第3周时升至(76.79±6.93)μmol/L,而后持续下降,至第6周时为(52.34±4.50)μmol/L;Ⅲ组大鼠停止注射地塞米松后血清一氧化氮水平逐渐上升,至第4周时为(26.32±2.93)μmol/L,其后维持在正常水平;Ⅳ组大鼠感染弓形虫后第3周时其一氧化氮水平升至(36.19±7.42)μmol/L,然后继续上升,至第5周时达最高至(63.60±16.32)μmol/L,然后下降;Ⅴ组大鼠的一氧化氮浓度则维持在从(13.96±2.50)μmol/L到(14.98±1.71)μmol/L之间的较低水平;Ⅵ组大鼠感染弓形虫后血清一氧化氮水平缓慢上升,至第5周时升至最高,为(58.89±21.70)μmol/L,然后下降。采用PCR的方法从Ⅳ组中的1只大鼠的脑组织扩增出SAG1片段,Ⅵ组有2只大鼠的脑组织扩增出SAG1片段,�
Objective To explore the effect of Toxoplasma gondii infection on the level of serum nitric oxide （NO） of rats and mice. Methods Spraque-Group of six Dawley female rats were divided into six groups. Group Ⅰ ：normal control. Group Ⅱ ： T.gondii infected. Group Ⅲ： subcutaneous injection with dexamethasone 2 mg/kg, every two days for two weeks, then stop. Group Ⅳ： subcutaneous injection with dexamethasone 2 mg/kg, every two days for two weeks, then infected with T.gondii tachyzoite and stop dexamethasone injection. Group Ⅴ： subcutaneous injection with dexamethasone 2 mg/kg, every two days during the experiment. Group Ⅵ： subcutaneous injection with dexamethone 2 mg/kg, every two days for two weeks, then infected with T.gondii tachyzoite and continued dexamethone injection. Sera were collected at weekly interval. Rats were killed at sixth week, and sera were collected. Infected rat brains were homogenized and inoculated into the abdominal cavity of mice. Specific SAG1 gene of toxoplasma in these brains was also amplified by PCR. Thirty-six Kunming mice were inoculated intraperitoneally with T.gondii RH strain. Mice were killed at 0, 1, 2, 3, 4, and 5 days post-infection and their sera were collected. The concentration of serum NO was determined by method of nitrate reductase. Results Concentration of serum NO in normal rat serum was （29.30± 2.77）μ mol/L. Two weeks after treatment with dexamcthasone, the concentration of serum NO in rats of group Ⅲ, Ⅳ, Ⅴand Ⅵ were （13.24±2.30）, （13.33±2.41）, （14.98±1.71）, and （14.55±2.99） μ mol/L, respectively. The level of serum NO in group Ⅱ was increased to （76.79±6.93） μ mol/L at the 3rd week after infection with T.gondii, and decreased to （52.34±4.50） μ mol/L at the 6th week. The level of serum NO in group Ⅲ was increased to （26.32± 2.93） μ mol/L at the 4th week, and the concentration was maintained at the normal level. In group Ⅳ, the concentration of serum NO was increased to （36.19±7.42）
出处
《热带医学杂志》
CAS
2006年第4期362-366,共5页
Journal of Tropical Medicine
基金
广东省重大科技攻关项目(No.A1090205)
广州市科技计划项目(No.2002Z-T24011)。
关键词
一氧化氮
地塞米松
弓形虫
大鼠
小鼠
nitric oxide
dexamethasone
Toxoplasma gondii
rats
mice
