摘要
目的:观察骨骼肌缺血再灌注损伤血清一氧化氮、诱导型一氧化氮合酶、内皮素1含量的变化及中药丹参的干预作用。方法:实验于2005-01/12在福建中医学院骨伤系实验室完成。实验分组:选用雄性SD大鼠84只,按随机数字表法分为丹参组、生理盐水组,每组42只,每组又分缺血再灌注10,20,30,40,50,60,90min7个时间点,每个时间点6只。实验方法:①制备大鼠左侧提睾肌缺血再灌注损伤模型。②丹参组于缺血2h时腹腔注射丹参注射液,生理盐水组给予相应剂量生理盐水;分别于缺血再灌注10,20,30,40,50,60,90min抽取腹主动脉血。实验评估:①采用硝酸还原酶法测定血清一氧化氮浓度。②采用比色法测定诱导型一氧化氮合酶活性。③采用放射免疫法测定内皮素1含量。结果:纳入大鼠84只,均进入结果分析。①一氧化氮、诱导型一氧化氮合酶的表达量:缺血再灌注10,20min两组一氧化氮、诱导型一氧化氮合酶的表达量无明显差异(P>0.05);缺血再灌注30,40,50,60,90min丹参组一氧化氮和诱导型一氧化氮合酶的表达量均高于生理盐水组[一氧化氮分别为(70.95±2.10),(68.21±2.23)μmol/L;(77.05±2.28),(72.20±1.56)μmol/L;(81.12±2.74),(74.60±1.90)μmol/L;(68.81±2.32),(62.03±2.80)μmol/L;(57.08±3.02),(46.77±3.01)μmol/L;诱导型一氧化氮合酶分别为(515.17±47.54),(459.78±37.27)μkat/L;(629.46±44.19),(499.37±29.46)μkat/L;(673.73±29.96),(584.77±58.48)μkat/L;(590.62±31.96),(507.78±31.82)μkat/L;(485.33±38.27),(378.64±38.04)μkat/L],差异有非常显著性意义(t=2.238,4.332,4.783,4.569,5.922;2.246,6.000,3.317,4.499,4.843,P<0.05,0.01)。缺血再灌注50min两组一氧化氮、诱导型一氧化氮合酶的表达量均达到最高峰,此后表达量开始下降。②内皮素1的表达量:缺血再灌注40min两组内皮素1的表达量均达到最高峰,此后表达量开始下降;缺血再灌注10min两组间内皮素1的表达量无明显差异(P>0.05),缺血再
AIM： To evaluate the effect of Danshen on the content changes of serum nitrogen monoxidum （NO）, inducible nitricoxide synthase （iNOS）, endothelin-1 （ET-1） of skeletal muscle during ischemia/reperfusion （I/R）.
METHODS： The experiment was conducted in the Department of Orthopaedics and Traumatology, Fujian College of Traditional Chinese Medicine from January to December 2005. Eighty-four male SD rats were randomly divided into Danshen group and saline group with 42 animals in each group. And each group was subdivided into I/R 10, 20, 30, 40, 50, 60, and 90 minutes groups with 6 rats in each group. ①Under anesthesia, the I/R models were established in the left cremaster muscles of rats. Danshen was intraperitoneally injected into Danshen group 2 hours after ischemia; the saline group was injected the same volume normal saline. ②The abdominal aorta blood was harvested after reperfusion 10, 20, 30, 40, 50, 60, and 90 minutes to detect the serum NO, iNOS and ET-1 by nitrate reductase, chromometry, and radioimmunity methods, respectively.
RESULTS： All 84 rats were involved in the result analysis. ①There was no obvious difference in the expressions of NO, iNOS between Danshen group and normal saline group reperfusion 10, and 20 minutes （P 〉 0.05）; the expression of NO, and iNOS in reperfusion 30, 40, 50, 60, and 90 minutes Danshen groups were significantly higher than that of normal saline group [NO： （70.95±2.10）, （68.21±2.23） μmol/L; （77.05±2.28）, （72.20±1.56）μmol/L; （81.12±2.74）, （74.60±1.90）μmol/L, （68.81±2.32）, （62.03±2.80） μmol/L; （57.08±3.02）, （46.77±3.01） μmol/L; iNOS： （515.17±47.54）, （459.78± 37.27） μkat/L; （629.46±44.19）, （499.37±29.46） μkat/L; （673.73±29.96）, （584.77±58.48） μkat/L; （590.62±31.96）, （507.78±31.82）μkat/L; （485.33±38.27）, （378.64±38.04） μkat/L; t =2.238, 4.332, 4.783, 4.569, 5.922, 2.246, 6,000, 3.317, 4.499, 4.643, P 〈 0.05, 0.01]. The expression
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第32期6440-6443,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金项目(30572401)
福建省自然科学基金项目(C0510023)
福建省引进高层次人才项目(1401)~~
