摘要
目的:探讨c-IAP1及其拮抗素Smac基因在白血病中的表达及对成人急性白血病(AL)患者预后的意义。方法:应用半定量逆转录-聚合酶链反应检测103例AL患者c-IAP1、Smac的mRNA表达水平,20例健康人为正常对照,K562、Kg-1α细胞株为阳性对照。结果:初治AL患者中c-IAP1、Smac的表达较正常对照者明显升高,2者的表达为高度正相关,治疗缓解后c-IAP1、Smac的表达明显下降,复发后c-IAP1、Smac:基因的表达又复升高。c-IAP1 mRNA和Smac mRNA在慢性粒细胞性白血病慢性期组的表达率和表达水平均高于正常对照组,但差异无统计学意义。初治AL患者中,c-IAP1、Smac表达阳性的患者缓解率均低于表达阴性的患者。结论:AL的发病可能与c-IAP1和Smac的高表达有关,而且是AL复发的高危因素;c-IAP1和Smac高表达的患者完全缓解率低,预后不良。
Objective：To investigate the expression of c-IAP1 and Smac in leukemia and the prognostic significance in adult acute leukemia（AL）. Method： The expression of c-IAP1 and Smac mRNA were measured in 103 leukemic adults by semi-quantity reverse transcription polymers chain reaction （RT-PCR）. Other 20 adults （as normal control, NC） and K562, Kg-1α cell lines （as positive control） were also measured. Result： The expression of c- IAP1 and Smac in de novo AL were higher than those in normal controls（NC）, while they decreased in complete remission patients. In replased patients, the expression of c-IAP1 and Smac increased again. The expression of c- IAP1 and Smac mRNA in CML-CP were higher than that of NC, but had no significance （ P 〉0.05）. In AL patients, the CR rate of c IAP1＋ and Smac^＋ cases were lower than c-IAP1- and Smac cases. Conclusion： Overex- pression of c-IAP1 and Smac may play a synergic role in the pathogenesis of leukemia and higher risk of relapse, c- IAP1 and Smac can serve as markers of poor prognosis in AL.
出处
《临床血液学杂志》
CAS
2006年第3期144-146,共3页
Journal of Clinical Hematology
