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酶定向进化的研究进展 被引量：3

Research in directed evolution of enzymes
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摘要 定向进化在改造酶的性质方面已得到广泛应用,各种建立突变库的方法不断涌现。对新近发展的几种突变技术(如寡核苷酸设计型装配重组技术ADO、非序列同源蛋白重组SHIPREC等)进行了简要地介绍与分类。与突变技术相对应的筛选方法也在逐渐改变和完善,这里仅介绍高通量筛选方面的一些最新进展。 Directed evolution has been widely used to change the characteristics of enzyme. The feasible methods for generating gene libraries such as ADO and SHIPREC were introduced in this article;and recent developments in high-throughput screen （HTS） for enzyme activity were discussed
作者 罗巅辉 方柏山
机构地区 华侨大学工业生物技术研究所
出处 《生物加工过程》 CAS CSCD 2006年第1期9-15,共7页 Chinese Journal of Bioprocess Engineering
基金 国家自然科学基金资助项目(20276026 20446004) 福建省自然科学基金资助项目(Z0516009) 福建省科技计划重点课题基金资助项目(2003I020)
关键词 定向进化 突变库 高通量筛选 directed evolution mutant library high-throughput screen （HTS）
分类号 Q55 [生物学—生物化学]
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参考文献35

  • 1[1]Linda GO,Wim J.Quax:Directed evolution:selecting today's biocatalysts[J].Biomolecular Engineering,2005,22:1-9. 被引量：1
  • 2[2]Reetz MT,Brunner B,Schneider T,et al.Directed evolution as a metbod to create enantioselective cyclohexanone monooxygenases for catalysis in Baeyer-Villiger reactions[J].Angew Chem Int Ed Engl,2004,43:4 075-4 078. 被引量：1
  • 3[3]Kim YW,Lee SS.Directed evolution of a glycosynthase from Agrobacteriumsp increases its catalytic activity dramatically and expands its substrate repertoire[J].J Biol Chem,2004,279:42 787-42 793. 被引量：1
  • 4[4]Bichet A,Bureik M,Lenz N,et al.The "Bringer" strategy:a very fast and highly efficient method for construction of mutant libraries by errorprone polymerase chain reaction of ring-closed plasmids[J].Appl Biochem Biotechnol,2004,117:115-122. 被引量：1
  • 5[5]Shao Z,Zhao H,Giver L,et al.Random-priming in vitro recombination:an effective tool for directed evolution[J].Nucl Acids Res,1998,26:681-683. 被引量：1
  • 6[6]Lee SH,Ryu EJ,Kang MJ,et al.A new approach to directed gene evolution by recombined extension on truncated templates (RETT)[J].J Mol Catal B-Enzym,2003,26:119-129. 被引量：1
  • 7[7]Zhao H,Giver L,Shao Z,et al.Molecular evolution by staggered extension process (StEP) in vitro recombination[J].Nat Biotechnol,1998,16:258-261. 被引量：1
  • 8[8]Coco WM,Levinson WE,Crist MJ,et al.DNA shuffling method for generating highly recombined genes and evolved enzymes[J].Nat Biotechnol,2001,19:354-359. 被引量：1
  • 9[9]Ostermeier M,Shim JH,Benkovic SJ.A combinatorial approach to hybrid enzymes independent of DNA homology[J].Nat Biotechnol,1999,17:1 205-1 209. 被引量：1
  • 10[10]Sieber V,Martinez CA,Arnold FH.Libraries of hybrid proteins from distantly related sequences[J].Nat Biotechnol,2001,19:456-460. 被引量：1

二级参考文献22

  • 1[1]Kolkman J A, Stemmer W P C. Directed evolution of proteins by exon shuffling. Nature Biotechnology, 2001, 19: 423～428 被引量：1
  • 2[2]Leung D W, Chen E, Goeddel D V. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique, 1989, 1: 11～15 被引量：1
  • 3[3]Ohuchi S, Nakano H, Yamane T. In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation. Nucleic Acids Res., 1998, 26: 4339～4346 被引量：1
  • 4[4]Suang R, Kawarasaki Y, Imaede T, et al. High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression. J. Mol. Biol., 2002, 318: 395～405 被引量：1
  • 5[5]Zhao H, Arnold F H. Optimization of DNA shuffling for high fidelity recombination. Nucleic Acids Res., 1997, 25: 1307～1308 被引量：1
  • 6[6]Shao Z, Zhao H, Giver L, et al. Random-priming in vitro recombination: an effective tool for directed evolution. Nucleic Acids Res., 1998, 26: 681～683 被引量：1
  • 7[7]Zhao H, Giver L, Shao Z, et al. Molecular evolution by staggered extension process (StMP) in vitro recombination. Nature Biotechnology, 1998, 16: 258～261 被引量：1
  • 8[8]Coco W M, Levinson W E, Crist M J, et al. DNA shuffling method for generating highly recombined genes and evolved enzymes. Nature Biotechnology, 2001,19: 354～359 被引量：1
  • 9[9]Lutz S, Ostermeier M, Benkovic S J. Rapid generation of incremental truncation libraries for protein engineering using α-phosphothioate nucleotides. Nucleic Acids Res., 2001, 29: e16 被引量：1
  • 10[10]Lutz S, Ostermeier M, Moore G L, et al. Creating multiple-crossover DNA libraries independent of sequence identity. Proc. Natl. Acad. Sci. USA, 2001, 98: 11248～11253 被引量：1

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生物加工过程
2006年 第1期

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