摘要
采用随机扩增多态性DNA(Random Amp lified Polymorph ism DNA,RAPD)技术,运用集群分离分析法(Bu lked SegregantAnalysis,BSA)研究与罗汉果性别连锁的分子标记。从90条随机引物的扩增产物中筛选出18条可能与性别连锁的RAPD标记。利用雌雄个体对这18条RAPD标记进行验证,发现引物S1431所扩增的约400bp谱带在8个雌性单株中都不出现,而在8个雄性单株中都出现,表明这是一条与雄性连锁的RAPD标记。
RAPD （ Random Amolified Polymorphism DNA） was employed to detect molecular markers linked to sex in S. grosvenorii by BSA （ Bulked Segregant Analysis）. 18 RAPD markers linked to sex were selected in BSA by screening 90 primers. Only the marker amplified by S1431 was present in all 8 male individuals tested while absent from all 8 female individuals tested. It showed that S1431 was a male RAPD marker linked to sex.
出处
《中药材》
CAS
CSCD
北大核心
2006年第4期311-313,共3页
Journal of Chinese Medicinal Materials
基金
广西科学基金项目(桂科基0342021)
广西农业科学院发展基金(2003003Z)
广西科学研究与技术开发计划基金(桂科能0443001-10)
关键词
罗汉果
性别连锁
RAPD
Siraitia grosvenorii
Sex linkage
RAPD
