摘要
目的探讨维吾尔族和汉族弱精子症病人精子mtDNA^(4977bp)缺失的相关性。方法本文采用聚合酶链反应(PCR)技术对60例标本分两组,维、汉各30例,其中精子活力正常标本(对照组)和弱精子症患者(观察组)各15例进行了精子线粒体DNA(mtDNA)缺失的研究。结果发现15例做内对照的维族精子活力正常人中仅有3例(20%)有mtDNA^(4977bp)缺失；而15例维吾尔族弱精子症患者中有10例(67%) mtDNA^(4977bp)缺失；两组4977bp缺失频率比较有显著性差异(P＜0．05)。15例做内对照的汉族精子活力正常标本中无mtDNA^(4977bp)缺失；15例汉族弱精子症患者中有12例(80%)mtDNA^(4977bp)缺失。两组4977bp缺失频率比较差异有显著性(P＜0．05)。同时发现维吾尔族男性不育症与汉族男性不育症mtDNA^(4977bp)缺失频率无显著性差异。结论维吾尔族和汉族mtDNA^(4977bp)缺失在弱精子症的发病中可能起重要作用。
Objective To investigate the relationship and differences for mtDNA^4977bp deletion of spermatozoa between the normal Hart and Uighur males with asthenozoospermia. Methods Using polymerease chain reaction （PCR） technique, the frequency of occurrence and properties of the mtDNA^4977bp deletion were determined in human spermatozoa with different motilities. According to the three criteria established by World Health Organization （1999）, 60 samples were divided into 4 groups. They were Uighur male with normal sperm （15 cases）, Uighur male with asthenozoospermia （15 cases）,Han nationality male with normal sperm （15 cases） and Han nationality male with asthenozoospermia （15 cases） and the sample in hot mutation region -4977bp of mtDNA were screened. Results The frequency of the presence of the 4977bp deletion in Uighur males with fertility was 20%. in Uighur male with infertility was 67 %： in Han males with fertility was 0%and in Han males with infertility was 80%. The frequency of the deleted mtDNA^4977bpthe spermatozoa with poor motility was significantly higher than those in the spermatozoa with good motility （P〈0.05）. In addition, there was no significantly differences on statistics （P〉0.05） between Uighur male and Han nationality male with infertility. Conclusion mtDNA^4977bp deletion may play an important role in the pathophysiology of some male infertility for both Han and Uighur male in Xinjiang.
出处
《中国男科学杂志》
CAS
CSCD
2006年第4期48-51,共4页
Chinese Journal of Andrology
基金
新疆医科大学校启动资金援助项目
