摘要
背景:血小板的参数可反映凝血活性,血小板α颗粒膜蛋白140可反映血小板的活化情况。糖尿病患者由于血糖、血脂代谢紊乱可导致各种并发症如血栓性疾病的发生。目的:分析2型糖尿病患者血小板参数及血小板α颗粒膜蛋白140的变化规律。设计:病例-对照观察。单位:华中科技大学同济医学院附属协和医院检验科和内分泌科。对象:选择2005-03/04在华中科技大学同济医学院附属协和医院内分泌科住院的2型糖尿病患者45例作为糖尿病组,选择同期健康体检者45例作为对照组,均自愿参加观察。方法:使用血细胞分析仪检测2型糖尿病患者的血小板数量、平均血小板容积、血小板容积分布宽度及大血小板比率的变化情况,应用酶联免疫吸附法观察血小板α颗粒膜蛋白140的变化,同时与健康体检者进行对比。主要观察指标:两组观察对象的血小板参数及血浆血小板α颗粒膜蛋白140含量。结果:纳入糖尿病组和对照组均45例,全部进入结果分析,无脱落。①两组观察对象血小板α颗粒膜蛋白140含量的比较:糖尿病组显著高于对照组眼(28.8±10.3),穴9.1±5.6雪μg/L(t=2.37,P<0.01)演。②两组观察对象血小板参数的比较:糖尿病组患者的血小板平均容积、血小板分布宽度及大血小板比率均显著高于对照组(t=2.02熏2.02熏2.02熏P<0.01),而血小板计数则显著低于对照组(t=2.15熏P<0.01)。结论:2型糖尿病患者的血小板平均容积、血小板分布宽度、大血小板比率及血浆血小板α颗粒膜蛋白140含量升高,而血小板计数降低,提示2型糖尿病患者存在着血小板凝血活性增高、血小板消耗增加的现象。
BACKGROUND： Parameters of platelet can reflect functions of coagulation. Granule membrane protein-140 （GMP-140）, which is also called P-Selectin, in or-granules of platelets can reflect activities of platelet. Dysfunction of blood sugar and blood lipid in diabetic patients can result in complications such as thrombotic diseases.
OBJECTIVE： To analyze the variation regulation of the platelet parameters and GMP-140 in patients with type 2 diabetes mellitus.
DESIGN： A case-control study. SETTING：Department of Clinical Laboratory and Department of Endocrinology, Union Hospital Affiliated to Tongii Medical College, Huazhong University of Science and Technology.
PARTICIPANTS： From March to April 2005, forty-five patients with type 2 diabetes mellitus, who were treated at the Department of Endocrinology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, were enrolled as diabetic group, and 45 healthy people, who were checked with health examination at the same period, were selected as control group. They all joined the experiment voluntarily.
METHODS： Changes of quantity of blood platelet, mean platelet volume （MPV）, platelet distribution width （PDW） and plateletdarge cell ratio （PLCR） were analyzed with blood cell counter. GMP-140 was measured by enzyme-linked immunosorbent assay （ELISA）, meanwhile, compared with those of the healthy people.
MAIN OUTCOME MEASURES： Contents of the parameters of platelets and GMP-140 of subjects in the two groups.
RESULTS： A total of 90 cases without drop out, 45 cases in the diabetic group and control group, respectively, were involved in the result analysis. ①Comparison of the content of GMP-140 in the two groups： It was significantly higher in the diabetic group than that in the control group [（28.8 ±10.3）,（9.1±5.6）pq/L（t=2.37,P 〈 0.01 ）1. ②Comparison of parameters of blood platelet of subjects of the two groups： The MPV, PDW and P-LCR in patients of the diabetic g
出处
《中国临床康复》
CSCD
北大核心
2006年第20期178-180,共3页
Chinese Journal of Clinical Rehabilitation
