摘要
目的研究NK-92细胞对人鼻咽癌细胞的体外杀伤活性，以及放射线照射后对其杀伤活性的影响。方法应用4h^51Cr释放法检测不同效靶比情况下体外培养的NK-92细胞对人鼻咽癌细胞CNE的杀伤活性，以K562细胞株作为对照。应用医用电子直线加速器对NK-92细胞进行照射处理（0、400cGy）后，检测其杀伤活性的变化。结果NK-92细胞0cGy照射组，效靶比较低时（1：1、5：1）对CNE细胞杀伤率为11％～24％，随效靶比升高，杀伤率随之上升，10：1时杀伤率为36％，20：1时杀伤率为42％；对于K562细胞，杀伤率为35％～70％。400cGy组照射后2d，NK-92细胞对CNE细胞不同效靶比的杀伤率与0cGy组相比稍有降低，其杀伤率为8％～38％，对K562细胞杀伤范围在31％～62％。结论在合适的效靶比情况下，NK-92细胞对人鼻咽癌细胞CNE具有杀伤作用。经放射线照射后对CNE细胞仍具有一定的杀伤活性。
Objective To study cytotoxic activity of NK-92 cells against human nasopharyngeal carcinoma cells and effect of irradiation on NK 92 cytotoxicity in vitro. Methods NK-92 cells were mixed with sl Cr-labeled CNE or K562 cells at different effector-to-target ratios for a 4 h^51Cr-release assay. On the other hand , NK-92 cells were irradiated at 0 and 400 cGy and tested after two days for cytotoxicity against K562 and CNE cells, Results NK-92 cells displayed cytotoxicity against CNE with a mean specific lysis of 36% and 42% at 10 ： 1 and 20 ： 1 E：T ratios in vitro, while these levels at 1 ： 1 （11%） and 5 ： 1 （24 %） E：T ratios were lower ; The cytotoxicity of NK-92 against K562 ranged from 35 % to ？0 %. NK-92 cells maintained up to 8 % -38 % cytotoxicity against CNE and 31% -62 % cytotoxicity against K562 when irradiated with 400 cGy after two days. Conclusion In vitro NK-92 ceils have cytotoxic activity against CNE cells at suitable E： T ratios, and substantial cytotoxicity can be retained up to irradiation dose of 400cGy.
出处
《中国临床保健杂志》
CAS
2006年第2期99-102,共4页
Chinese Journal of Clinical Healthcare
基金
国家863计划资助课题(2002AA216151)
关键词
鼻咽肿瘤
杀伤细胞
天然
肿瘤细胞
培养的
细胞毒性
免疫
Masopharyngeal neoplasms
Killer cells, mtural
Tumor cells, cultured
Cytotoxicity, immunologic
