摘要
目的研究放射线照射后NK92细胞对人卵巢癌细胞杀伤活性及其成瘤性的影响。方法应用医用电子直线加速器对NK92细胞进行照射处理(800cGy),以体外培养的人卵巢癌细胞株HO8910为靶细胞,以细胞株K562作为对照,应用4h51Cr释放法检测不同效靶比情况下体外培养NK92细胞的杀伤活性。建立人卵巢癌SCID鼠肿瘤模型,观察输注经照射后的NK92细胞对荷瘤鼠的治疗效果。结果(1)体外实验NK92细胞未照射组,效靶比较低时(11、51)对HO8910细胞的杀伤率为39%～45%,随效靶比升高,杀伤率上升,101时杀伤率升为59%,201时杀伤率仅上升了6%;对于K562细胞,杀伤率在58%～71%之间。照射后2d,800cGy组NK92细胞对HO8910细胞不同效靶比的杀伤率比0cGy组略有降低,总体杀伤率在33%～58%之间;对于K562细胞,杀伤率在39%～49%之间。照射后NK92细胞数量进行性下降,第5天细胞死亡;(2)动物实验分别在接种后30、40、50d观察治疗组与对照组肿瘤体积,结果显示,分别减小了78%、56%、20%(P<0.01)。结论NK92细胞对人卵巢癌细胞具有较高的杀伤活性,经放射线照射后仍保留一定的杀伤活性,作为一种生物治疗手段治疗卵巢癌行之有效。
Objective:To study the cytotoxic activity of NK 92 cells against human ovarian cancer cells in vitro and in vivo and the effect of irradiation on NK 92 cytotoxicity.Methods:NK 92 cells were mixed with 51 Cr labeled HO 8910 and K562 cells at different effector to target ratios for a 4h 51 Cr release assay.On the other hand, NK 92 cells were irradiated at 0 and 800 cGy and tested after 2 days for cytotoxicity against HO 8910 cells.In animal experiments, human ovarian carcinoma in a SCID mouse model was devised, and the potential of irradiated NK 92 as immunotherapy for ovarian carcinoma was evaluated.Results:In vitro,unirradiated NK 92 cells displayed high cytotoxicity against HO 8910 with a mean specific lysis of 59% and 65% at 10:1 and 20:1 E:T ratios, while these levels at 1:1(39%) and 5:1(45%) E:T ratios were lower.The cytotoxicity of NK 92 against K562 ranged from 58% to 71%. NK 92 cells maintained up to 33%～58% cytotoxicity against HO 8910 when irradiated with 800cGy after 2 days. In vivo treatment of mouse with irradiated NK 92 cells, tumor size was reduced 78% as compared with control on day 30 after injection and 56% on day 40 and 20% on day 50(P<0.01).Conclusion:NK 92 cells are highly cytotoxic to human ovarian carcinoma both in vitro and in vivo in a mouse model.Irradiated NK 92 cells may be a potentially effective immunotherapeutic modality in human ovarian carcinoma.
出处
《现代妇产科进展》
CSCD
2004年第4期251-254,共4页
Progress in Obstetrics and Gynecology
基金
国家高技术研究发展计划(863计划)资助课题(2002AA216151)
关键词
杀伤细胞
天然
卵巢肿瘤
细胞毒性试验
免疫
SCID鼠
肿瘤移植
疾病
模型
动物
Killer cells,natural
Ovarian neoplasms
Cytotoxicity tests,immunologic
SCID mouse
Neoplasm transplantation
Disease models,animal
