摘要
背景:在传统方剂五苓散的基础上加用田七、丹参等组成制剂健神利水I号主要对脑水肿“瘀、水”进行治疗;加用石菖蒲、夏枯草等组成的制剂醒脑消肿胶囊主要针对脑水肿“火、痰、窍闭”进行治疗。目的:观察健神利水I号与醒脑消肿胶囊对血管源性脑水肿的疗效差异。设计:随机区组设计,空白对照实验。单位:广西中医学院第二附属医院神经内科实验室。材料:实验于2001-03/04在广西中医学院第二附属医院神经内科实验室完成。选用新西兰大耳白兔32只,雄性,兔龄7个月。方法:①采用自体无肝素新鲜血注入法造成脑水肿动物模型。用区组随机法将动物分为4组:空白对照组(不做任何干预)、模型组(造模后不做任何干预)、健神利水Ⅰ号组(造模后按11.5mL/kg剂量灌服健神利水Ⅰ号煎剂,健神利水Ⅰ号煎剂由三七15g,丹参15g,茯苓10g,猪苓10g,泽泻10g,白术10g,桂枝10g组成,广西中医学院第二附属医院药房提供,200mL/袋)和醒脑消肿胶囊组(造模后灌服按380mg/kg用烧开的蒸馏水制成11.5mL/kg醒脑消肿胶囊混悬液,胶囊批准文号南药(96)05079),由夏枯草15g,石菖蒲15g,茯苓10g,猪苓10g,泽泻10g,白术10g,桂枝10g组成,含生药13.33g/粒,由广西中医学院第二附属医院制剂室提供],每组8只动物。每组动物再分为12和24h两个时间段进行观察,每个时间点4只。②分别于干预12和24h后以空气栓塞法处死动物。计算脑系数(全脑质量/体质量×100%),脑含水量=[(湿重-干重)/湿重×100%],脑组织中伊文思蓝含量[测定吸光度(A值)×标准液中微克/1个A值×(25mL/湿脑重)]。③正态性检验用矩法,组间比较用F检验,组间两两比较用q检验,时间段(同组)内比较用t检验,方差不齐用秩和检验。主要观察指标:实验12和24h各组脑系数、脑含水量、伊文思蓝含量变化比较。结果:兔32只均进入结果分析。①实验12h:健神利水Ⅰ号组和醒
BACKGROUND： On the basis of traditional herb wuling san, tianqi and danshen are added to make a＇preparation, jianshen lishui Ⅰ, for treating blood stasis and edema; shichangpu and xiakucao are added to make a preparation, xingnao xiaozhong capsule, for treating fire, sputum and qiaobi in cerebral edema.
OBJECTIVE： To observe the differences between.jianshen lishui Ⅰ and xingmao xiaozhong capsule on treating vascular-derived cerebral edema.
DESIGN： Randomized grouping design and blank controlled study. SETTING; Laboratory of Neurology, Second Affiliated Hospital of Guangxi College. of Traditional Chinese Medicine.
MATERIALS： The experiment was completed in the Laboratory of Neurology, Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine from March to April 2001. Totally 32 male rabbits with 7 months old were selected in the study.
METHODS： ① Animal models with cerebral edema were established with self-fresh blood injection method. According to grouping method, animals were divided randomly into 4 groups： blank control group （without intervention）; model group （without intervention after modeling）; jianshen lishui ① group （11.5 mL/kgjianshen lishui I was perfused after modeling; jianshen lishui I consisted of 15 g sanqi, 15 g danshen, 10 gfuling, 10 g zhuling, 10g zexie, 10 g baizhu and 10 g guizhi, and provided by Dispensary of the Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine, 200 mL/bag） and xingnao xiaozhong capsule group [after modeling, 11.5 mL/kg xingnao xiaozhong suspension was made by 380 mg/kg boiled distilled water;, certification： southern medicine （96） 05079; consisting of 15 g xiakucao, 15 g shichangpu, 10 gfuling, 10 g zhuling, 10 g zexie, 10 g baizhu and 10 g guizhi, 13.33 g raw materials per capsule; provided by Agent Department of the Second Affiliated Hospital of Guangxi Col- lege of Traditional Chinese Medicine] with 8 in each group. Animals in each group were divided into 12 and 24 hours su
出处
《中国临床康复》
CSCD
北大核心
2006年第15期168-170,共3页
Chinese Journal of Clinical Rehabilitation
基金
广西壮族自治区卫生厅基金资助项目(桂卫字9925)~~
