摘要
目的：采用超临界CO2从云南红豆杉枝叶中萃取紫杉醇。方法：以云南红豆杉枝叶为原料，先以纯CO2做萃取溶剂，除去原料中的脂类，再加入甲醇为夹带剂提取紫杉醇。用HPLC法检测萃取物中紫杉醇的含量。结果：紫杉醇的最佳萃取条件为：红豆杉枝叶粉碎至直径0．6-0．8mm，苹取压力34MPa，萃取温度40℃，甲醇为夹带剂。结论：SPE法较传统溶剂法，提取率有所提高，且使用有机试剂少，有利于环境保护，耗时少，生产周期短，效率高。
AIM： To study the supercritical-CO2 fluid extraction of taxol from branches and needles of Taxus Yunnanensis, METHODS： Pure CO2 was used to remove lipid in branches and needles of Taxus Yunnanensis and then methanol was used as modifier to extract taxol. A HPLC method was developed for the determination of taxol content in extracts. RESULTS： Taxol could be extracted from branches and needles of Taxus Yunnanensis which was grinded to powder of diameter about 0.6-0.8 mm at 34 MPa and 40 ℃ when methanol was used as modifier. CONCLUSION： Comparing with traditional solvent extraction method, supercritical-CO2 fluid extraction can improve extraction yield of taxol and protect natural environment because of its less wasted solvent and short time.
出处
《中成药》
CAS
CSCD
北大核心
2006年第4期480-482,共3页
Chinese Traditional Patent Medicine
关键词
超临界CO2萃取
云南红豆杉
紫杉醇
supercritical-CO2 fluid extraction
Taxus Yunnanensis
taxol
