摘要
目的探讨阿魏酸钠在整体水平对结肠炎大鼠结肠巨噬细胞功能的影响及其机制。方法建立大鼠免疫性结肠炎模型。阿魏酸钠(SF)灌肠用药21d后检测结肠组织MDA、NO、PGE2含量,SOD、IL_1,TNF_α、MPO活性及NF_κBp65表达水平。结果阿魏酸钠(200,400,800mg/kg)灌肠后呈剂量依赖型降低模型组大鼠的MDA、NO、PGE2含量,IL_1、TNF_α、MPO活性及NF_κBp65表达水平,同时升高SOD活性。结论SF可减弱结肠炎大鼠结肠活化巨噬细胞的生物活性,缓解结肠炎症反应,机制可能与抑制NF_κB表达有关。
Objective To explore the effects of sodium ferulate（SF） on function of macrophages in colonic tissue of the colitis rats in vivo and its mechanism. Methods The immunological colitis model of rats was produced. SF was used intracolonically for 21 days. The contents of MDA, NO, PGE2, the activity of SOD, IL-1, TNF-α and myelopexoxidase（ MPO）, and the expression level of NF-κB p65 in colonic tissue of the rats were detected. Results SF（200,400,800 mg/kg） decreased the contents of MDA, NO, PGE2 ,the activity of IE-1 ,TNF-α and MPO, and the expression level of NF-α Bp65 while increased the activity of SOD in colonic tissue of the colitis rats in a dose-depended manner. Conclusion SF can restrain the activity of activated colonic macrophages and relieve the colonic inflammation reaction in vivo in colitis rats, which may be related with the suppression of NF-κB expression.
出处
《胃肠病学和肝病学杂志》
CAS
2006年第2期116-118,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
湖北省科技攻关项目(NO:2001AA308B)
