摘要
目的:观察灌胃给予含糖、电解质、微量元素的运动饮料对于负重游泳训练SD大鼠恢复运动疲劳的作用及机制。方法:实验于2005-02/04在解放军第二军医大学长海医院中心实验室完成。①将48只7周龄SD大鼠随机分为对照组(n=12),40g/L葡萄糖组(n=18),饮料组(n=18)。将每组大鼠在水温平均(30±1)℃,水深50cm,直径50cm的陶瓷缸中用细线绳固定5g重铅块进行负重游泳训练。②游泳前实验大鼠灌胃,每只每次4mL液体,分别为生理盐水、40g/L葡萄糖、自行设计配制的运动饮料,灌胃时间每只平均15s。③第1次负重游泳30min后取出大鼠快速进行第2次灌胃。用红外线取暖器对鼠笼保温休息20min,立即进行第2次负重游泳50min,取出后快速进行第3次灌胃。以同样方式保温休息20min,进行第3次负重游泳至力竭。④力竭判断标准为大鼠沉入水中超过10s,无法浮出水面,视为力竭,记录力竭时间。结果:实验纳入48只大鼠全部完成训练,均进入结果分析,中途无脱落。①3组大鼠负重游泳持续时间的均值分别为对照组(124.00±52.11)min、40g/L葡萄糖组(138.61±83.05)min、饮料组(204.50±75.20)min。②3组大鼠力竭时间的区间分别为对照组(59～240)min、40g/L葡萄糖组(30～316)min、饮料组(108～393)min。③3组大鼠游泳至力竭时间,对照组与40g/L葡萄糖组无统计学差异,与饮料组差异有显著性意义(P=0.016);40g/L葡萄糖组与饮料组统计学差异有显著性意义(P=0.03)。结论:①含糖、电解质、微量元素的运动饮料能够明显延长大鼠负重游泳至力竭时间。②保持运动前、中、后机体的水、电解质及能量平衡是快速消除运动疲劳的有效手段。
AIM： To study the effect and mechanism of a new beverage containing glucose, electrolytes and trace elements by gastric perfusion on the recovery in loaded-swimming rats.
METHODS： The study was carried out from February to April 2005 in the Central Laboratory of Changhai Hospital, Second Military Medical University of Chinese PLA. ① Forty-eight 7-weeks SD rats were randomly divided into three groups： control group （n=12）, 40 g/L glucose group （n=18） and beverage group （n=18）. All the rats were trained to swim bearing a 5 g lead brick （fastened around the tail using a thin rope） in a 50 cm diameter ceramic vat with water depth of 50 cm and water temperature about （30±1） ℃. ②Before swimming, all the rats were treated with gastric perfusion administrated with each beverage （4 ml each rat and the beverage of the three groups were saline, 40 g/L glucose and new beverage respectively. The average administration time was 15 s）. ③Immediately after the administration, the rats were trained to swim for thirty minutes. The rats were administered again in the same gastric perfusion. All the rats rested for 20 minutes （the cages were kept warm by infrared warmers）, followed by a second time of swimming for 50 minutes, Then, all rats were administered for the third time in the same order and rested for another 20 minutes. A third time swimming training was followed and the swimming time until exhaustion of the third swimming was recorded. ④The criterion for exhaustion was： The rat sank into water for over 10 s and could not reach the water surface.
RESULTS： A total of 48 experimental rats were involved in the result analysis, without drop out. ①The average swimming time and the range until exhaustion in rats of the 3 groups were （124.00±52.11） minutes in the control group, （138.61±83.05） minutes in the 40 g/ L glucose group and （204.50±75.20） minutes in the beverage group, respectively. ②The swimming time until exhaustion of rats were （59-240）minutes i
出处
《中国临床康复》
CSCD
北大核心
2006年第16期114-116,共3页
Chinese Journal of Clinical Rehabilitation
