摘要
目的探讨肉桂酸对提升小鼠运动耐力的影响。方法通过建立力竭性游泳来建立小鼠力竭性运动模型,比较安静对照组、运动训练组和运动给药组小鼠力竭游泳持续时间,血浆中乳酸(LAC)含量来探讨肉桂酸对小鼠的耐力提升;检测肝脏组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)和过氧化酶(CAT)的含量来探讨肉桂酸的抗氧化能力,通过Western blot方法检测肉桂酸对小鼠股四头肌中Na+/K+-ATP酶、Ca2+/Mg2+-ATP酶表达水平来进一步研究其对小鼠运动能力提升的影响。结果肉桂酸显著延长小鼠的游泳时间;运动给药组与安静对照组比较:血浆LAC含量虽然有所升高,但差异无统计学意义(P>0.05),肝脏组织匀浆中SOD和CAT显著下降(P<0.05),MDA显著上升(P<0.01),CAT虽下降但差异无统计学意义(P>0.05),Na+/K+-ATP酶和Ca2+/Mg2+-ATP酶的表达水平显著下降(P<0.01);运动训练组与安静对照组比较,血浆LAC含量显著升高(P<0.01),肝脏组织匀浆中SOD和CAT显著下降(P<0.01),MDA显著上升(P<0.01),Na+/K+-ATP酶和Ca2+/Mg2+-ATP酶的表达水平显著降低(P<0.01);运动给药组与运动训练组比较,血浆LAC含量显著降低(P<0.01),肝脏组织匀浆中SOD和CAT显著上升(P<0.01),MDA显著下降(P<0.01),CAT显著下降(P<0.01),Na+/K+-ATP酶和Ca2+/Mg2+-ATP酶的表达水平显著降低(P<0.05)。结论肉桂酸具有良好的抗氧化能力和抗疲劳能力。
Objective To investigate the effects of cinnamic acid to enhance the exercise endurance in mice. Methods Through the exhaustive swimming to establish mice exhaustive exercise model, the mice were divided into control group, exercise control group and exercise cinnamic group（n = 10）, the mice exhaustive swimming time were comparted,blood lactic acid（LAC） lew els were used to explore the endurance of cinnamic acid in mice; the super oxide dismutase （SOD）, malondialdehyde （MDA） and peroxidase （CAT） content in liver homogenates were used to detect mice endurance enhancement and antioxidant capacity of cinnamic acid, and expression of Nan/K＋ -ATP enzyme, Ca2＋/Mg2＋ -ATP enzyme on mice quadriceps by Western blot to further study its effect on improving exercise capacity in mice. Results Cinnamic acid significantly prolonged the swimming time of mice; Exercise cinnamic group compared with control group：although the LAC content in plasma elevated,the difference was not statistically significant（P〉0.05）, SOD and CAT in liver homogenate was significantly decreased（P〈0, 05）, MDA increased significantly（P〈 0.01）, CAT was decreased but with no statistically significant （P^0.05）, the expression level of Nan/K＋ -ATP enzyme and Ca2＋ / Mg2＋-ATP enzyme decreased significantly（P〈0. 01）. Exercise control group compared with control group., the LAC content in plasma increased significantly（P〈0.01）, SOD and CAT in liver homogenate was significantly decreased（P〈0.01）, MDA increased significantly（P〈 0.01）, expression level of Na＋/K＋-ATP enzyme and Ca2＋/enzyme Mg2＋-ATP decreased significantly （P〈 0.01） ;Exercise cinnamic group exercise control group..the LAC content in plasma was significantly reduced （P〈0.01）, SOD and CAT in liver tissue significantly increased （P〈 0.01 ）, MDA decreased significantly （P 〈 0.01 ）, CAT decreased significantly （P〈 0.01）, the expression level of Nan/K＋ -ATP enzyme and Ca2＋/Mg2＋ -ATP en
出处
《重庆医学》
CAS
北大核心
2015年第35期4932-4935,共4页
Chongqing medicine
关键词
肉桂酸
运动耐力
抗氧化
cinnamic acid
endurance exercise
antioxidant
