摘要
目的:观察电离辐射对caspase-3蛋白表达的影响。方法:采用体外传代培养的小鼠EL-4淋巴瘤细胞为实验材料;采用单克隆抗体免疫荧光标记,FACScan流式细胞仪(FCM)检测蛋白表达的变化;采用PI荧光标记及FCM测定细胞凋亡的变化;量效实验,观察0.5、1.0、2.0、4.0及6.0 Gy X射线照射后24 h EL-4细胞caspase-3蛋白表达及细胞凋亡的变化;时效实验,观察4.0 Gy照射后2、4、8、12、24、48及72 h EL-4细胞caspase-3蛋白表达的变化。结果:量效实验表明,0.5、2.0、4.0及6.0 Gy X射线照射后24 h EL-4细胞中caspase-3蛋白表达均明显增高,与假照射对照组比较,差异具有显著性(P<0.05或P<0.01)。0.5、1.0及4.0 Gy照射后24 h EL-4细胞凋亡明显增高,与假照射对照组比较,差异具有显著性(P<0.05或P<0.01)。时效实验表明,4.0 Gy照射后8、12及24 h EL-4细胞中caspase-3蛋白表达均明显增高,与同时间点假照射对照组比较,差异具有显著性(P<0.01或P<0.001)。结论:电离辐射可诱导EL-4细胞caspase-3蛋白表达增高。同时诱导EL-4细胞凋亡。
Objective To investigate the effect of ionizing radiation on caspase-3 protein expression of EL-4 cells. Methods Mouse lymphoma EL-4 cell line was used. Flow cytometry （FCM） was used to examine caspase-3 protein expression and apoptosis. For dose-effect experiment, caspase-3 protein expression and apoptosis were measured 24 h after X-irradiation with doses of 0.5, 1.0, 2.0, 4. 0 and 6.0 Gy. For time course experiment, caspase-3 protein expression was measured at 2, 4, 8, 12, 24 and 72 h after 4.0 Gy X-irradiation. Results In dose-effect experiment, it was demonstrated that the caspase-3 protein expression of EL-4 cells was increased significantly 24 h after X-irradiation with the doses of 0.5, 2.0, 4.0 and 6.0 Gy compared with sham-irrad （P〈0.05 or P〈0.01）. It was also showed that apoptosis of EL-4 cells was induced significantly 24 h after X-irradiation with the doses of 0.5, 1.0 and 4.0 Gy compared with sham-irrad （P〈0. 05 or 〈0.01）. In time course experiment, the result showed that the caspase-3 protein expression of EL-4 cells was increased significantly 8, 12 and 24 h after 4.0 Gy X-irradiation compared with sham-irrad （P〈0. 01 or P〈0. 001）. Conclusion Caspase-3 protein expression and apoptosis in EL-4 cells could be induced by X-rays.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2006年第2期179-181,共3页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(30270346)
