摘要
目的为探索器官移植后急性排斥反应的一种快速可靠的诊断方法。方法先暴露和游离受体的吻合段血管,再获取供心以缩短移植心脏冷缺血时间;保留供者的心脏及右上肺,将其胸主动脉与受者的腹主动脉间断端侧吻合;开放血流时,先开放远心端再间断开放近心端,同时按摩心脏至心脏搏动规律、有力,其色泽恢复红润,右上肺亦充盈良好。结果A组(n=20)成功16只,动脉吻合口出血2只、心脏复跳失败1只、吻合口狭窄1只;B组(n=20)成功17只,2只死于吻合口出血,1只死于吻合口狭窄。A组手术成功率为80%,B组为85%(P>0.05)。结论(1)先暴露并游离好受体血管吻合段,再获取供心,可以缩短移植心冷缺血时间。(2)边灌注边获取的方法利于快速而又完整的获取供心。(3)在切取和植入的过程中一定要注意低温保护。(4)利用腹主动脉段进行移植,使手术方便易行。左心做功的大鼠腹部心脏移植模型简单、安全、实用,因其左心参与循环而使血流动力学更接近生理状态,是进行心脏移植及血流动力学研究的较理想模型。
Objective To improve the techniques for establishment of a stable and reproducible animal model of heart transplantation in rat for further research on acute rejection. Methods To expose the recipient＇ s abdominal arteries at first. Then to obtain the donor＇s heart and upper right lung. The donor＇s ascending aorta was anatomized with recipient＇ s abdominal aorta and then blood flowed through donor＇s organ. Results 80 heterotopic heart transplantations were performed, 4 rats died of anastomotic bleeding, 2 died of the stenosis of anastomosis and 1 died of the failure of heart rebound, and the total successful rate was above 80 %. Conclusion The modified surgical techniques and careful operative management can contribute to better success in heart transplantation in rats.
出处
《中国实验动物学报》
CAS
CSCD
2006年第1期56-58,共3页
Acta Laboratorium Animalis Scientia Sinica
关键词
心脏移植
移植物排斥
模型
动物
Heart transplantation
Graft rejection
Models, animal
