摘要
目的评价免疫治疗后荷瘤小鼠骨髓来源的树突状细胞(DC)体外激发T细胞增殖及分泌细胞因子的功能。方法采用腹腔注射激发型4-1BB抗体联合DC主动免疫治疗荷瘤小鼠3。H-TdR掺入试验检测免疫治疗后荷瘤小鼠骨髓来源DC体外激发T细胞增殖的能力,ELISA法检测DC刺激T细胞分泌IL-2、IFN-γ及IL-10的水平。结果荷瘤小鼠骨髓前体细胞产生的DC激发T细胞增殖及分泌IL-2、IFN-γ的能力下降,但经过免疫治疗后,这类DC激发T细胞增殖及分泌IL-2、IFN-γ的能力均得到改善,而且免疫治疗效果越好,改善就明显。结论荷瘤小鼠经免疫治疗后,其骨髓前体细胞来源DC的免疫功能得到改善。
Objective To evaluate the function of bone marrow derived DC of tumor bearing mice after immunotherapy. Methods Tumor bearing mice were immunized with DC vaccine plus injection of agonistic anti-4-1BB monoclonal antibody. The proliferation of T cells primed with bone marrow derived DC of tumor bearing mice after immunotherapy was tested by ^3H-TdR incorporation. ELISA was employed to detemine the levels of IL-2, IFN-γ and IL-10 secreted by DC primed T cells. Results Bone marrow derived DC of tumor bearing mice was less efficient in stimulating the proliferation of T cells and IL 2 and IFN-γ secretionmade by T cells. After immunotherapy, the proliferation of cells and IL-2 and IFN-γ secretionmade by T cells were enhanced. Conclusion The function of bone marrow derived DC of tumor bearing mice after immunotherapy was ameliorated.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2006年第1期1-3,共3页
Suzhou University Journal of Medical Science
基金
国家自然科学基金资助项目(30271205)
江苏省临床免疫学重点实验室基金资助
