摘要
目的研究类风湿关节炎(RA)患者滑液(SF)及外周血(PB)中嗜中性核粒细胞巨噬细胞炎性蛋白1α(MIP-1α)的表达水平,探讨其在RA发病中的作用。方法利用梯度密度离心法从RA患者SF及PB和健康对照者PB中分离嗜中性核粒细胞、单个核细胞,应用双抗夹心酶联免疫吸附试验 (ELISA),测定RA患者SF及PB上清中MIP-1α水平,通过反转录聚合酶链反应(RT-PCR)法测定MIP-1α在RA患者SF、PB和健康对照者PB中嗜中性核粒细胞mRNA的表达,并与RA疾病活动性指标和SF中单个核细胞数进行相关分析。结果 RA患者SF中嗜中性核粒细胞MIP-1α水平明显高于PB和健康对照者,且与RA疾病活动性指标和SF中单个核细胞数明显相关。MIP-1α mRNA在RA患者SF嗜中性核粒细胞有明显表达。加入肿瘤坏死因子经24 h培养后RA患者SF嗜中性核粒细胞MIP-1α分泌明显增加。结论 SF嗜中性核粒细胞MIP-1α表达可诱导RA患者关节局部和系统性炎症,作为C-C趋化因子MIP-1α可促进单个核细胞由PB进入关节滑膜及组织,导致滑膜增生、血管翳形成、关节破坏。
Objective To study the level of macrophage inflammatory protein 1α （MIP-1α） in the synovial fluid and peripheral blood neutrophils in rheumatoid arthritis （RA） and discuss it＇s role in RA pathogenesis. Methods Neutrophils and monocytes were isolated from samples of synovlal fluid and peripheral blood of RA patients and healthy controls respectively by Ficoll-Hypaque. The MIP-1α level was detected by ELISA and the MIP-1α mRNA was detected by RT-PCR. The relationship between MIP-1α in synovial fluid neutrophils, RA disease activity and monocytes counts in synovial fluid was analysed. Results The MIP-1α level of synovial fluid neutrophils in RA patients was significantly higher than that in the peripheral blood and healthy controls. The level of MIP-1α in synovial fluid neutrophils was correlated with RA disease activity and monocytes counts in synovial fluid. MIP-1α mRNA in synovial fluid neutrophils was overexpressed in RA. MIP-1α level in RA synovial fluid neutrophils was significantly increased after 24 hours incubation with TNF. Conclusion The expression of MIP-1α in synovial fluid neutrophils may induce local and systemic inflammation in RA. MIP-1α, as a C-C chemokine, may promote the infiltration of monocytes from peripheral blood into svnovial Joints and tissues, leading, to svnovial vroliferation, oannus formation and articular destruction.
出处
《中华风湿病学杂志》
CAS
CSCD
2006年第3期133-136,共4页
Chinese Journal of Rheumatology
基金
国家教育部归国人员启动基金资助项目[教外司留(2002)24]
辽宁省科技攻关计划课题基金资助项目(2003225007-6)
辽宁省教育厅高等学校科学研究资助项目(202013151)
关键词
类风湿关节炎
巨噬细胞炎性蛋白质1
中性白细胞
滑液
外周血
Arthritis, rheumatoid
Macrophage inflammatory protein-1
Neutrophils
Synovial fluid
Peripheral blood
