摘要
目的研究大川芎丸对大鼠缺血脑组织中血管内皮细胞生长因子(VEGF)表达的影响。方法采用大鼠大脑中动脉缺血(MCAO)模型,将20只SD大鼠随机分成治疗组和对照组,每组各10只,分别于术后每天予以大川芎丸提取物4.13g/kg或相应剂量生理盐水灌胃给药,72h后行神经功能评分后处死,取脑组织作免疫组化染色检测VEGF蛋白表达变化。结果大川芎丸治疗组神经功能评分(1.5±0.71)低于对照组(2.3±0.82),差异有统计学意义(P=0.032);大川芎丸治疗组VEGF表达(19.13±1.52)高于对照组(14.83±0.94),差异有统计学意义(P=0.000)。结论中药大川芎丸可上调大鼠缺血脑组织中VEGF表达,并可能通过此机制起到保护作用。
Objective To study the effect of Dachuanxiongwan on the expression of vascular epithelial growth factor in the rats after focal cerebral ischemia and reperfusion. Methods The ischemia and reperfusion model was established by blocking the middle cerebral artery of the rats with a nylon thread for two hours. Twenty male Sprague-awley rats with middle cerebral artery occlusion （MCAO） were randomly divided into two groups. One of the group of rats were administered with DCXW, 4. 13 g/kg· d, twice a day for 3 dais by garaging, while other group were given the same volume of saline as a control. Seventy-wo hours after reperfusion, the neuropathological function of the rats were evaluated according to Longa. The rats were then sacrificed and the samples of cerebrum were processed and embedded in paraffin and cut into sections. The expression of VEGF was determined by immunocytochemistry. Results The score of the neuropathological function of the DCXW treated group（1.54± 71） was lower than that of the control group （2. 34± 82, P= 0. 032）. More VEGF-ositive cells were found in the rats treated with DCXW than those in the control group（P= 0. 000）. Conclusion DCXW upgrades the expression of VEGF and may have a positive effect on the cerebrum following ischemia and reperfusion.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第2期246-249,共4页
Journal of Sichuan University(Medical Sciences)
关键词
缺血性脑血管病
线栓法大脑中动脉栓塞模型
大川芎丸
血管内皮细胞生长因子
Ischemic cerebrovascular disease Middle cerebral artery occlusion （MCAO） Dachuanxiongwan （DCXW） Vascular epithelial growth factor（VEGF）
