摘要
利用溶菌酶对中等脱乙酰度壳聚糖的选择性降解,将其与高脱乙酰度壳聚糖以不同比例混合浇铸成膜。考察了降解过程中材料的失重和溶液还原糖含量的变化,用傅立叶红外光谱分析了混合膜的成分改变和降解过程中膜的变化,并用扫描电镜观察了混合膜降解后的形态变化。结果表明,壳聚糖混合膜中的中等脱乙酰度壳聚糖被溶菌酶选择性的降解,而且当混合膜中该组分含量为0.5时,降解后膜表面及内部均产生了互相贯穿的纳米尺寸的孔,剩余的高脱乙酰度成分表现为纳米尺寸的纤维。该方法通过壳聚糖的选择性酶解原位成孔,为制备纳米结构的多孔壳聚糖膜提供了一个新颖的途径。
A novel approach for the in situ pore formation in chitosan membranes based on the selective enzymatic polymer degradation. Moderately deacetylated chitosan （MDC） was blended with highly deacetylated chitosan （HDC） in certain ratios in solution and the solution was cast to obtain chitosan membranes. The degradation was conducted in phosphate buffer solution by monitoring weight loss, reducing sugar, FTIR and SEM. The results revealed that the component of MDC in chitosan blend membranes was selectively degraded by lysozyme and then removed from the membranes, and when containing half MDC, the blend membranes underwent obvious degradation and the resulted membranes exhibited a nano-scale porous structure. This method was expected as potential candidate for preparation of porous chitosan membranes in biomedical use.
出处
《功能材料》
EI
CAS
CSCD
北大核心
2006年第3期462-464,469,共4页
Journal of Functional Materials
基金
国家自然科学基金资助项目(2002CB713804)
国家高技术研究发展计划(863计划)资助项目(2001AA326020)
关键词
壳聚糖
降解
膜
孔
chitosan
degradation
film
porosity
