摘要
目的通过检测糖皮质激素受体-α(GR-α)mRNA在鼻息肉和鼻腔正常黏膜中的定量表达,探讨糖皮质激素在鼻腔局部用药的吸收途径。方法采用荧光定量逆转录PCR(FQ-RT-PCR)方法检测101例鼻息肉和31例鼻腔正常黏膜中GR-αmRNA的定量表达。结果GR-α在鼻腔正常黏膜(135.4±5.25)×104拷贝/μg中比鼻息肉(23.5±12.1)×104拷贝/μg中表达高,两组之间有显著性差异。结论鼻息肉组织中GR-αmRNA低表达提示糖皮质激素不经鼻息肉黏膜吸收,而是经过鼻腔的正常黏膜吸收。
OBJECTIVE To detect the expression of the Glucocorticoid receptor- α （GR-α ） mRNA in nasal polyp and normal nasal mucosa and investigate the route of nasal drug administration in patients of nasal polyp. METHODS The expression of GR-α mRNA in 101 samples of nasal polyps and 31samples of normal nasal mucosa was examined by using Fluorescent quantitative PCR .RESULTS The level of GR-α mRNA in normal nasal mucosa （135.4 ± 5.25）×10^4 copy / U g were significantly higher than that in nasal polyps （23.5 ± 12.1）×10^4copy/μg. CONCLUSION Because of the low expression of GR-α mRNA in nasal polyps, the route of nasal drug administration in patients with nasal polyps may be in the part of normal mucosa, not in the mucosa of nasal polyps of the nasal cavity.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2006年第1期39-41,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
广东省科技计划资助项目(2003C30309)
关键词
鼻息肉
糖皮质激素类
受体
糖皮质激素
荧光定量PCR
投药
鼻内
Nasal Polyps
Glucocorticoids
Receptors, Glucocorticoid
Fluorescent Quantitative PCR （FQ-RT-PCR）
Administration, Intranasal
