摘要
目的对细粒棘球蚴谷胱苷肽S-转移酶基因进行克隆和序列分析。方法从包虫病患者体内获取细粒棘球蚴原头蚴提取总RNA,根据国外细粒棘球蚴谷胱苷肽S-转移酶基因的已知序列设计一对引物,采用RT-PCR技术扩增,将PCR产物纯化后,将其克隆到pGEM-T载体后进行序列测定以及生物信息学分析。结果用RT-PCR成功扩增出细粒棘球蚴中国大陆株谷胱苷肽S-转移酶基因,测序表明该基因开放阅读框为660bp,与已发表基因核苷酸序列相比,同源性为99%。结论成功克隆了细粒棘球蚴中国大陆株谷胱苷肽S-转移酶基因序列,为其进一步的研究奠定基础。
Objective To clone and analyze sequence of Glutathione S - transferase（GST） gene of Echinococcus granulosus. Methods Total RNA was extracted from protoscoles of cysts from hurnam origin. The specific primers were designed according to published nucleotide sequence in the Genebank database. The GST gene of Echinococcus granuLosus was amplified by RTPCR and cloned into pGEM- T vector for sequencing and analyzing. Results A cDNA sequence with an open reading frame of 660bp had been amplified successfully by RT - PCR. Comparision of the DNA and amino acid sequence deduced from cDNA with the published GST gene sequence of Echinococcus granulosus in the Genebank revealed 99% homology. Conclusion The GST gene sequence of Echinococcus granulosus was cloned successfully and its biological study could be performed on the basis.
出处
《宁夏医学院学报》
2006年第1期4-6,共3页
Journal of Ningxia Medical College
基金
国家自然科学基金资助项目(30260105)
