摘要
目的探讨冷冻心肌梗死模型的特点以及卫星细胞移植在治疗心梗中的作用。方法将卫星细胞移植到大鼠心梗中央区,用免疫组化、共聚焦免疫荧光双标染色及电镜等多种方法研究移植细胞的增殖分化情况。结果用直径5 mm的铝棒冷冻大鼠左心室前壁15 s,可致左心室重量43%的心肌缺血,17%的心肌梗死,在2周左右可发展成透壁心梗。将卫星细胞移植到大鼠心梗中央区后,细胞大量成活,在早期增殖旺盛,2周后分化形成较多的多核肌管,有的发育成肌纤维,形成了完整的肌节,但移植细胞和宿主心肌细胞之间没有形成缝管连接。结论冷冻心梗模型是研究心肌梗死的良好模型。骨骼肌卫星细胞移植可部分修复心肌梗死区。
Objective To find the features of cryo-injured infarcted model and the therapeutic effects of skeletal satellites cells（SCs） on myocardial infarction （MI）. Methods SCs were implanted into the center of cryo-injured myocardium. Immunohistochemistry, confocal immunofiuorescence double-labelling staining and transmission electron microscopy were used to study the state of SCs. Results After cryo-injured frontal wall of the left ventricle（LV） for approximately 15 s by aluminium rod with 5 mm in diameter, it made 43% weight of LV ischemia and 17% weight of LV infarcted. At 2nd week, injured area occured as transmural infarct. Mter transplantation, the implanted ceils survived in large numbers. The transplanted ceils proliferated remarkably at early phase and differentiated into many multinuclear myotubes after 2 weeks. Some of them developed into muscle fibers with better sarcomeres, but the gap junction was not formed between implanted ceils and cardiac myocytes. Conclusions Cryo-injured infarcted model is a proper model to study myocardial infarction. Skelatal satellite cell transplantation will repair morphological structure partly of myocardial infarction area.
出处
《基础医学与临床》
CSCD
北大核心
2006年第2期161-165,共5页
Basic and Clinical Medicine
基金
河北省教育厅项目(2005117)
关键词
卫星细胞
移植
心肌梗死
satellite cell
transplantation
myocardial infarction
