摘要
目的观察P19细胞植入大鼠心肌后成活、分化及对大鼠心功能的影响。方法SD大鼠30只,随机分为移植组(n=20)和对照组(n=10)。用液氮冷冻方法建立心肌梗死模型,梗死后立即将培养的P19细胞植入心肌梗死区及周边区,8周后观察植入细胞的成活、分化,并通过血流动力学各项指标评价细胞移植对心功能的改善情况。结果移植组免疫组化染色GATA-4、α-肌节肌动蛋白(-αsarcomeric actin)及肌细胞生成蛋白(myogenin)在成活移植细胞呈阳性,左室收缩压(LVSP)、左室等容期压力最大变化速率(±dp/dtmax)明显高于对照组(P<0.01),左室舒张末期压(LVEDP)明显低于对照组(P<0.01)。移植组有1例检测心功能时发现有偶发室性早搏。结论P19细胞移植入心肌梗死区后能够成活并向心肌分化,可明显改善心肌梗死后大鼠的心功能;P19细胞可以作为研究心肌梗死细胞移植疗法的模型细胞。
Objective To investigate the differentiation of transplanted P19 cells and improving of heart function using MI model of rat. Methods Sprague-Dawley rats （n = 30） were separated randomly into two groups： treatment group （n = 20） and control group （n = 10）. Left ventricle of the animal＇s heart was cryoinjured to create a transmural infarct. Then, P19 cells were transplanted into the transmural lesion and border lesion immediately. At eighth week after transplantation, the living cells and differentiation of transplanted cells were observed morphologicly and heart function of two groups were measured. Results Living transplanted cells were found in the lesion of MI, and the cells shown positive of GATA-4,α-sarcomeric actin and myogenin in immunohistochemical staining. Compared with control group, LVEDP was lower in treatment group （P 〈 0.01 ）, LVSP and ＋ dp/dtmax were higher in treatment group （P 〈 0.01 ）. Conclusions After P19 cell transplantation, transplanted cell can living in transmural lesion and have a tendency to differentiate to muscle cell in MI model. P19 cell transplantation may be a novel approach to improve ventricular function in transmural infarct. P19 cell is a feasible modal cell to study the cellular therapy for heart failure.
出处
《基础医学与临床》
CSCD
北大核心
2005年第12期1156-1160,共5页
Basic and Clinical Medicine
关键词
P19细胞
移植
心肌梗死
心功能
大鼠
P19 cell
transplantation
myocardial infarction
heart function
