摘要
目的了解谷胱甘肽硫转移酶P1(GSTP1)、M1(GSTM1)基因多态性在广东梅州地区人群中的分布规律。方法采用聚合酶链式反应-限制性酶切片段长度多态性(PCR-RFLP)方法和以β-Globin为内对照的双重PCR法分别检测广东省梅州地区512名健康客家居民GSTP1和GSTM1基因型。结果调查人群GSTP1基因3种基因型GSTP1A/A、GSTP1A/G、GSTP1G/G分布频率分别为69.1%,28.2%,2.7%;GSTM1基因缺失型分布频率为62.1%;GSTM1基因在饮酒人群与不饮酒人群中的分布差异有统计学意义(P<0.05),GSTP1基因在吸烟与不吸烟人群中的分布差异有统计学意义(P<0.01),吸烟人群中GSTP1A/A基因型分布频率较低(62.5%),GSTP1A/G基因型分布频率较低(29.2%),GSTP1G/G基因型分布频率较高(8.3%)。GSTM1基因型分布与高血压家族史有相关性(OR=1.868,95%CI1.119～3.119)。结论GSTP1基因多态性在广东梅州地区吸烟与不吸烟客家人群中分布有差异,GSTM1在广东梅州地区饮酒与不饮酒客家人群中分布有差异,GSTM1基因型分布与高血压家族史有相关性。
Objective To investigate the distribution of genetic polymorphisms of human glutathione-S-tnmsferase P1 and M1 （GSTP1 and GSTM 1） in Hakka population of Meizhou area in South China. Methods Co-amplifying GSTM1 and β-Globin gene to detect the absence or presence of the GSTM1 gene, polymerase chain reaction-restric.tion fragment length polymorphism （PCR-RFLP） were used to identify the genotypes ofGSTP1 gene,and the data were analyzed with SPSS10.0 software. Results The GSTP1 and GSTM1 genetic polymorphisms were examined in 512 samples. The frequency of null genotype of GSTM1 gene was 62.1%, while the frequency of GSTP1 A/A, GSTP1 A/G and GSTP1 G/G were 69.1%, 28.2% and 2.7% respectively, and the GSTP1 genetic polymorphism distribution was in accordance with the Hardy-Weinberg equilibrium rule. A significant difference was observed in GSTM1 genetic polymorphisms between drinkers and non- drinkers. The drinkers had higher frequency of absence GSTM1 gene. There was also a significant difference in the GSTP1 genetic polymorphisms between smoking group and non- smoking group. Compared with non-smokers, the smokers had a higher frequency of mutate genotype of GSTP1 gene and lower frequency of wild genotype. However there were no differences shown on the frequency of the GSTP1 genotypes in the aspect of cumulative tobacco consumption. Logistic regression analysis results showed that there was a weak association between the GSTM1 genetic polymorphisms and family history of hypertension. Conclusion There are significant differences in the GSTP1 genetic polymorphisms among different smoking status and in GSTM1 genetic polymorphisms between drinkers and non-drinkers. A weak association has been observed between the GSTM 1 genetic polymorphisms and family history of hypertension.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第2期112-115,共4页
Journal of Environment and Health
基金
广东省自然科学基金资助项目(015042)
