摘要
目的 研究亚甲基四氢叶酸还原酶 (MTHFR)基因A12 98C多态性及其和烟酒茶嗜好相互作用与胃癌易感性的关系。方法 在上消化道癌高发区淮安市进行病例 对照研究 (胃癌 15 6例 ,人群对照 2 2 8例 ) ,调查研究对象的生活习惯 ,采用PCR RFLP技术检验研究对象的MTHFR基因型。结果 ( 1)胃癌组和对照组中MTHFRAl2 98C变异型等位基因 (AC +CC)携带者分别占 2 8.1%和 3 3 .4% ,其差异无统计学显著性。 ( 2 )吸烟和饮酒习惯与MTHFR 12 98变异基因型在升高胃癌发生的危险性中有明显的交互作用 ,这种交互作用符合相乘模型 ,( 3 )不饮茶作为独立的危险因素增加胃癌发生的危险性。结论 MTHFRAl2 98C基因多态性影响吸烟、饮酒和饮茶习惯与胃癌发生之间的关系。调查生活习惯的同时检测MTHFRAl2
Objective To investigate the relationship between polymorphisms of methylennetetrahydrofolate reductase A1298C (MTHFR) and susceptibility of gastric cancer.Methods 156 patients with gastric cancer and 228 healthy subjects in Huaian city of Jiangsu province were comparatively investigated.The epidemiological data were collected,and DNA of peripheral blood leukocytes was obtained from all of the subjects.MT HFRAl298C genotypes were detected by PCR-RFLP method.Results (1) The frequency of METHFRAl298C variantgenotypes (AC+CC) in patients and control group was 28.1% and 33.4% respectively,there was no significant difference between the two groups.(2) There was a significant interaction among habits of smoking and alcohol drinking and MTHFRAl298C variant genotype in gastric cancer,and this interaction fitted multiplicative model.(3) An increase of gastric cancer risk was found in individuals who had no habit of tea drinking.Conclusion There is a interaction between MTHFRAl298C genotypes and habits of smoking and alcohol drinking in developing gastric cancer.
出处
《河北医药》
CAS
2004年第11期851-853,共3页
Hebei Medical Journal
基金
日本文科省国际学术研究癌症特别研究经费资助
项目编号 :0 80 4 2 0 1 5
