摘要
背景与目的:肽基脯氨酰顺反异构酶Pin1在人类多种肿瘤中过表达,Pin1过表达可增强CyclinD1表达,在肿瘤发生发展中起重要作用。本研究拟探讨宫颈癌细胞株及宫颈上皮组织中Pin1、CyclinD1的表达及其临床意义。方法:应用RT-PCR、Westernblot法检测HeLa、SiHa、C33a、Caski细胞中Pin1和CyclinD1基因蛋白表达,免疫组化法检测10例正常宫颈、21例宫颈上皮内瘤样病变、57例浸润癌组织中Pin1和CyclinD1的表达状况,评价其临床意义。结果:HeLa、SiHa、C33a、Caski细胞存在不同程度Pin1mRNA及蛋白过表达(P<0.05)。正常宫颈、宫颈上皮内瘤样病变、浸润癌组织中Pin1蛋白表达逐渐增强,分别为0%、47.62%、64.91%(P<0.05)。Pin1蛋白过表达与宫颈癌临床分期、病理分级、淋巴结转移无关(P>0.05);但宫颈腺癌组织中Pin1表达明显高于鳞癌(P<0.05)。Pin1过表达与CyclinD1表达呈显著正相关(P<0.05)。结论:Pin1在宫颈癌细胞株及宫颈癌组织中存在mRNA及蛋白水平上的过表达,Pin1过表达与CyclinD1表达密切相关,Pin1和CyclinD1异常表达可能参与宫颈癌的发生发展。
BACKGROUND ＆ OBJECTIVE： Peptidyl-prolyl cis/trans isomerase Pin1 is prevalently overexpressed in human cancers. Up-regulation of Pin1 elevates the expression of Cyclin D1, and plays an important role in tumorigenesis and tumor progression expression and clinical significance of cell lines and cervical epithelial tissues This study was to investigate the Pin1 and Cyclin D1 in cervical cancer METHODS; The expression of Pin1 and Cyclin D1 in cervical cancer cell lines HeLa, SiHa, C33a and Caski were detected by reverse transcription-polymerase chain reaction （RT-PCR） and Western blot. Their expression in 88 samples of cervical tissues, including 10 samples of normal cervix, 21 samples of cervical intraepithelial neoplasia （CIN）, and 57 samples of invasive cervical cancer, were detected by immunohistochemistry. RESULTS, The mRNA and protein levels of Pin1 were significantly higher in HeLa, SiHa, C33a, and Caski cells than in normal cervical epithelial tissues （P〈0.05）. The expression of Pin1 increased progressively along with the disease process from normal cervix to CIN, and to invasive cervical cancer （0%, 47.62%, 64.91%, P〈0.05）. Pin1 expression had no relation to disease stage （FIGO）, pathologic grade, and pelvic lymph node metastasis status （P〉0.05）. The positive rate of Pin1 was significantly higher in cervical adenocarcinoma than in cervical squarnous cell carcinoma （100% vs. 60.0%, P〈0.05）. In cervical cancer tissues, the overexpression of Pin1 was positively correlated to that of Cyclin D1 （P〈0.05）. CONCLUSIONS; Pin1 is overexpressed in HeLa, SiHa, C33a and Caski cell lines as well as in cervical cancer tissues. The overexpression of Pin1 is closely related to Cyclin D1 expression in cervical cancer. The aberrant expression of Pin1 and Cyclin D1 might contribute to tumorigenesis of cervical cancer.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2006年第3期367-372,共6页
Chinese Journal of Cancer
基金
国家"973"重点基础科学基金项目(No.2002CB513107)
国家自然科学基金项目(No.30271358)~~
