摘要
背景:颈动脉窦存在压力感受器,是离子对血压调节最敏感的部位,在血压的调节中起到重要的作用,但不同离子通过颈动脉窦对血压的调节作用还不十分清楚。目的:观察用不同浓度、不同离子浸泡颈动脉窦后血压的变化。设计:自身对照实验。单位:解放军第四军医大学基础部教学实验中心。材料:实验于2000-12/2001-06在解放军第四军医大学基础部教学实验中心机能实验室完成。选取纯种新西兰兔18只,实验动物标准为二级,雌雄不限,体质量(2.0±0.2)kg,由解放军第四军医大学动物实验中心提供。方法:采用随机数字表法将白兔分为Na+组、K+组和Ca2+组,每组6只。将家兔麻醉后,气管插管,分离两侧颈动脉,一侧分离出颈动脉窦,另一侧进行血管插管并记录血压,用加液枪于颈动脉窦外分别由低浓度到高浓度滴加Na+、K+和Ca2+溶液,将颈动脉窦完全浸泡;记录每次加液前血压的基础值和加液后血压变化的峰值。主要观察指标:家兔颈动脉血压的基础值和加液后血压变化的峰值。结果:0.15,1.5mol/L的高浓度Na+加液后血压为(97±12),(83±17)mmHg,与基础值(106±14),(105±12)mmHg比较有显著的降血压作用(t=2.946,P<0.05);0.4mol/L的高浓度K+有显著的降血压作用[(106±12),(64±13)mmHg,(t=13.496,P<0.01)],其他浓度的K+降血压作用不明显(P>0.05);0.07mol/L的低浓度Ca2+加液后血压为(113±16)mmHg,与基础值(103±12)mmHg比较有显著的升血压作用(t=-3.627,P<0.01)。结论:Na+、K+和Ca2+可以通过直接作用于颈动脉窦调节血压。高浓度的Na+、K+具有降压作用,低浓度的Ca2+具有升压作用,这可能是血压调节的一种重要机制。
BACKGROUND： There are pressure sensors in carotid-sinus, which are very sensitive to blood＇pressure regulated by ions and play an important role in the regulation of blood pressure. But it is yet not very clear how different ions regulate the blood pressure through pressure sensors in carotidsinus.
OBJECTIVE： To observe the effect of different ions at various concentrations outside the carotid-sinus.
DESIGN： Self-control experiment.
SETTING： Preclinical Experiment Center, Fourth Military Medical University of Chinese PLA. MATERIALS： The experiment was accomplished in the Preclinical Experiment Center, Fourth Military Medical University of Chinese PLA from December 2000 to June -2001. Totally 18 New Zealand pure strain rabbits were provided by the Animal Experimenting Center of Fourth Military Medical University of Chinese PLA. They were standard grade Ⅱ, of either gender and body mass was （2.0±0.2） kg.
METHODS： The rabbits were divided into Na^＋, K^＋ and Ca^2＋ groups according to random numbers, and each group consisted of 6 rabbits. After anaesthesia, tracheal intubatton was performed on the rabbit, and bilateral carotid arteries were separated with carotid-sinus separated on one side and vessel intubatton performed in the other side for blood pressure measurement. Then various concentrations of Na^＋, K^＋ and Ca^2＋ions were added outside the carotid-sinus with the pipette to make the carotid-sinus completed immersed in the ion solutions. The basal blood pressure and the peak value after ions addition were recorded respectively.
MAIN OUTCOME MEASURES： The basal blood pressure and the peak value after ions addition. RESULTS： After Na^＋ （0.15, 1.5 mol/L） was added the blood pressure was （97±12）, （83±17） mm Hg. h was decreased significantly compared with the basal value （106±14）, （105±12） mm Hg （t=2.946, P 〈 0.05）. K^＋ （0.4 moL/L） decreased the blood pressure significantly [（106±12）, （64±13） mm Hg, （t =13.496, P 〈 0.01�
出处
《中国临床康复》
CSCD
北大核心
2006年第8期182-183,共2页
Chinese Journal of Clinical Rehabilitation
