摘要
目的:检测卵巢上皮性癌中17号染色体上野生型p53基因缺失与HER-2基因的扩增情况并探讨其临床意义。方法:分别以不同颜色荧光标记的p53、HER-2基因和17号染色体着丝粒为双色探针组,应用间期双色荧光原位杂交(FISH)技术检测54例卵巢上皮性癌间期细胞核中p53基因、HER-2基因和17号染色体的拷贝数及其相对比例,以确定其缺失或扩增,并统计分析其临床意义。结果:54例卵巢上皮性癌中p53基因缺失38例(70.4%);HER-2基因扩增14例(25.9%);17号染色体多倍体有35例(64.8%)。p53基因缺失与17号染色体多倍体呈正相关(χ2=12.116,P<0.001),与HER-2基因的扩增无关(χ2=0.00,P=1.00)。p53基因缺失与卵巢上皮性癌患者肿瘤分期及体积大小无关(P>0.05),而与血CA125水平及淋巴结转移有关(P<0.05);术后2年存活率p53基因缺失患者显著低于无缺失患者(81.3%,χ2=4.186,P=0.047)。结论:p53基因高频缺失和HER-2基因扩增,可能与部分卵巢上皮性癌的发生发展有关。
Objective： To investigate the deletion of p53 gene and amplification of HER-2 oncogene at chromosome 17 in epithelial ovarian carcinoma（EOC） and the clinical significance. Methods： Interphase dual fluorescence in situ hybridization （FISH） was applied to detect the ratio of the number of p53 gene copy or HER-2 oncogene copy to that of chromosome 17 copy, to determine the p53 gene deletion and HER-2 oncogene amplifcation in nuclei prepared from 54 surgical specimens of EOC. Statistical analysis for their clinical signifcance was performed. Results： Loss of p53 gene and amplifcation of HER- 2 oncogene were detected in 38 （70.4%） and 14 （25.9%） of 54 EOC, respectively, including 6 cases with high and 8 with low copy amplification. Two （3.7%） of 54 EOC showed simultancously p53 gene deletion and HER-2 oncogene amplifcation. 54.8% （35/54） of EOC in polysomy 17 positively correlated with p53 gene deletion （χ^2= 12.116, P 〈 0.001 ）. No correlation between p53 gene loss and HER- 2 oncogene amplifcation was found （χ^2= 0.00, P= 1.00）. Loss of p53 gene was related with serum CA125 level and the metastasis of pelvic lymph nodes （ P 〈 0.05）, but no relation with tumor size and FIGO staging （ P 〉 0.05）. The postoperative 2-year survival rate （57.9%） of EOC patients with p53 gene deletion was lower than that （81.3%） ofwithout p53 gene loss （χ^2= 4.186, P=0.047）. Conclusions： There is a high kequency of p53 gene deletion and a low frequency of HER-2 oncogene amplification in EOC, which might be involved in initiation and development of a subeet of EOC.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2006年第2期89-91,i0001,共4页
Journal of Practical Obstetrics and Gynecology
关键词
卵巢肿瘤
基因
染色体
荧光
Ovarian neoplasms
Genes
Chromosomes
Fluorescence
