期刊文献+

Rho激酶在缺氧性肺动脉平滑肌细胞中的表达 被引量:2

The expression of Rho kinase of pulmonary arterial smooth muscle cells exposed to hypoxia
下载PDF
导出
摘要 目的:探讨缺氧对大鼠肺动脉平滑肌细胞(PASMC)增殖及其中Rho激酶表达的影响。方法:分别在常氧和缺氧12、24和48 h条件下体外培养大鼠PASMC,应用噻唑蓝(MTT)比色法检测细胞增殖情况,流式细胞术测定细胞周期,同时用蛋白印迹杂交(W estern b lot)检测Rho激酶的表达情况。结果:PASMC比色吸光度A值缺氧12 h增大,24 h最高,48 h下降但仍高于常氧对照组;流式细胞术分析细胞周期,G2/M期细胞缺氧组均显著高于常氧组;W estern b lot结果分析各缺氧组Rho激酶表达明显高于常氧组。结论:缺氧诱导PASMC增殖,促使细胞进入有丝分裂期,同时,Rho激酶表达的增强可能是缺氧诱导PASMC增殖的重要机制之一。 Objective : To explore the effects of hypoxia on expression of Rho kinase and proliferation of rat pulmonary arterial smooth muscle cells(PASMC). Methods: Cultured PASMC were exposed to normoxia and hypoxia for 12,24 and 48 h. Viability of PASMC was examined by MTT assay. The cell cycle was analyzed by flow cytometry. Westem blot was used to assess the levels of Rho kinase after primarily cultured rat PASMC were exposed to hypoxia for 12,24 and 48 h. Results: The result of MTT was increased in PASMC to hypoxia for 12 h compared with PASMC in normoxia. After exposure of PASMC to hypoxia for 24 h, the result of MTT peaked, then declined in 48 h, but still higher than that of PASMC when exposed to normoxia. Analysis of cell cycle indicated that the ability of cell proliferation increased significantly in PASMC exposed to hypoxia compared with normoxia. Rho kinase level was higher in PASMC exposed to hypoxia compared with normoxia. Conclusion: Hypoxia stimulates proliferation of PASMC and promotes PASMC into the phase of mitosis. Expression of Rho-kinase is increased in proliferated PASMC induced by hypoxia and the increased expression of Rho-kinase maybe one of important pathogenesis in hypoxia-induced proliferation of PASMC.
出处 《医学研究生学报》 CAS 2006年第2期111-113,共3页 Journal of Medical Postgraduates
基金 国家自然科学基金资助项目(批准号:30370624)
关键词 缺氧 肺动脉 平滑肌细胞 RHO激酶 Hypoxia Pulmony artrey Smooth muscle cell Rho kinase
  • 相关文献

参考文献6

二级参考文献37

  • 1李丰,车东媛,刘绍春,邓仲端.缺氧对肺动脉内皮细胞PDGF基因及PDGF-B链蛋白表达的影响[J].中华病理学杂志,1995,24(3):139-142. 被引量:16
  • 2王培勇,孙秉庸.培养的肺动脉内皮细胞生长及生化特性的初步观察[J].第三军医大学学报,1995,17(3):212-213. 被引量:19
  • 3[1]Tsai JC,Wang H,Perrella MA et al.Induction of cyclin A gene expression by homocysteine in vascular smooth muscle cells[J].J Clin Invest,1996,97(1):146-153. 被引量:1
  • 4[2]Blackstock WP,Weir MP.Proteomics:quantitative and physical mapping of cellular proteins[J].Trends Biotechnol,1999,17(3):121-127. 被引量:1
  • 5[4]Ross R.The smooth muscle cell.Ⅱ.Growth of smooth muscle in culture and formation of elastic fibers[J].J Cell Biol,1971,50(1):172-186. 被引量:1
  • 6[5]Bollag DM,Edelstein SS.Protein methods[M].2nd,New York:Wiley-Liss,Inc.1996.50-55. 被引量:1
  • 7[7]Hodges-Garcia YK,Madigan N,Horwitz LD.Primary human vascular smooth muscle cell culture enhanced by human umbilical cord serum[L].In Vitro Cell Dev Biol Anim,1998,34(5):364-366. 被引量:1
  • 8[8]Chen C,Halkos ME,Surowiec SM et al.Effect of homocysteine on smooth muscle cell proliferation in both cell culture and artery perfusion culture models[J].J Surg Res,2000,88(1):26-33. 被引量:1
  • 9Janssen B,Rindermann M,Barth U,et al.Linkage analysis in a large family with PPH:genetic heterogeneity and a second PPH Locus on 2 q31-32.Chest,2002,121(Suppl):54S-56S. 被引量:1
  • 10Christman BW,McPherson CD,Newman JH,et al.An imbalance between the excretion of thromboxane and prostacyclin metabolites in pulmonary hypertension.N Engl J Med,1992,327:70-75. 被引量:1

共引文献31

同被引文献26

  • 1张敏,吴壮,徐军.损伤气道上皮组织转化在气道重塑中的作用[J].医学研究生学报,2004,17(11):966-969. 被引量:4
  • 2邱慧,张德平.γ干扰素后期干预对大鼠肺纤维化影响[J].医学研究生学报,2006,19(10):888-891. 被引量:7
  • 3解伟光.烧伤患者能量消耗的基本变化[J].医学研究生学报,2007,20(3):269-271. 被引量:8
  • 4Sem HP. The myofibroblast in pulmonary fibrosis [ J ]. Chest, 2002,122 : S286-289. 被引量:1
  • 5Zhang H, Gharaee-Kermani M, Zhang K, et al. Lung tlbroblast alpha-smooth muscle actin expression and contractile phenotype in bleomycin-induced pulmonary fibrosis [ J ]. Am J Pathol, 1996,148 (2) :527-537. 被引量:1
  • 6Darby I, Skalli O, Gabbiani G. Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing[ J ]. Lab Invest, 1990,63( 1 ) :21-29. 被引量:1
  • 7Bustin M. Regulations of DNA-dependent activities by the functional motifs of the high-mobility-group chromosomal proteins [J]. Mol Cell Biol, 1999,19(8) :5237-5246. 被引量:1
  • 8Shimizu RT, Blank RS, Jervis R, et al. Distinct protein targets for signals acting at the c-fos serum response dement[ J]. J Biol Chem, 1995,270 ( 13 ) :7631-7643. 被引量:1
  • 9Mack CP, Owens GK. Regulation of smooth muscle alpha-actin expression in vivo is dependent on CArG elements within the 5' and first intron promoter regions [ J ]. Circ Res, 1999,84 (7): 852 -861. 被引量:1
  • 10Ge H, Roeder RG. The high mobility group protein HMG1 can reversibly inhibit class II gene transcription by interaction with the TATA-binding protein [ J ]. J Biol Chem, 1994,269 ( 25 ) : 17136-17140. 被引量:1

引证文献2

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部