摘要
目的:探讨缺氧对大鼠肺动脉平滑肌细胞(PASMC)增殖及其中Rho激酶表达的影响。方法:分别在常氧和缺氧12、24和48 h条件下体外培养大鼠PASMC,应用噻唑蓝(MTT)比色法检测细胞增殖情况,流式细胞术测定细胞周期,同时用蛋白印迹杂交(W estern b lot)检测Rho激酶的表达情况。结果:PASMC比色吸光度A值缺氧12 h增大,24 h最高,48 h下降但仍高于常氧对照组;流式细胞术分析细胞周期,G2/M期细胞缺氧组均显著高于常氧组;W estern b lot结果分析各缺氧组Rho激酶表达明显高于常氧组。结论:缺氧诱导PASMC增殖,促使细胞进入有丝分裂期,同时,Rho激酶表达的增强可能是缺氧诱导PASMC增殖的重要机制之一。
Objective : To explore the effects of hypoxia on expression of Rho kinase and proliferation of rat pulmonary arterial smooth muscle cells(PASMC). Methods: Cultured PASMC were exposed to normoxia and hypoxia for 12,24 and 48 h. Viability of PASMC was examined by MTT assay. The cell cycle was analyzed by flow cytometry. Westem blot was used to assess the levels of Rho kinase after primarily cultured rat PASMC were exposed to hypoxia for 12,24 and 48 h. Results: The result of MTT was increased in PASMC to hypoxia for 12 h compared with PASMC in normoxia. After exposure of PASMC to hypoxia for 24 h, the result of MTT peaked, then declined in 48 h, but still higher than that of PASMC when exposed to normoxia. Analysis of cell cycle indicated that the ability of cell proliferation increased significantly in PASMC exposed to hypoxia compared with normoxia. Rho kinase level was higher in PASMC exposed to hypoxia compared with normoxia. Conclusion: Hypoxia stimulates proliferation of PASMC and promotes PASMC into the phase of mitosis. Expression of Rho-kinase is increased in proliferated PASMC induced by hypoxia and the increased expression of Rho-kinase maybe one of important pathogenesis in hypoxia-induced proliferation of PASMC.
出处
《医学研究生学报》
CAS
2006年第2期111-113,共3页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目(批准号:30370624)
关键词
缺氧
肺动脉
平滑肌细胞
RHO激酶
Hypoxia
Pulmony artrey
Smooth muscle cell
Rho kinase