摘要
目的:小鼠肝癌微波消融后检测树突状细胞归巢,探讨热消融治疗激发抗肿瘤免疫效应的可能性。方法:建立C57BL/6J小鼠Hepa1-6肝癌皮下移植瘤模型,不同温度微波消融后,瘤内注射荧光标记的未成熟树突状细胞(PKH26-DC),检测PKH26-DC归巢的数量、成熟情况及对淋巴细胞的刺激作用。结果:(65±5)℃和(90±5)℃消融组未发现PKH26-DC归巢和CCR7表达。(50±5)℃消融组和未消融组比较,归巢DC的数量为32±8vs21±6,CCR7表达率为100%vs90%,100倍视野下淋巴细胞形成免疫突触的数量为8-12簇vs4-6簇,每簇内淋巴细胞数目为12-25vs3-10。结论:肝癌热消融能促进树突状细胞成熟和归巢,刺激淋巴细胞免疫应答,但消融温度须适当。
AIM： To investigate the homing effect of immature dendritic cells （iDC） after injection of iDC into the mouse hepatoma treated by microwave ablation and the possibility of stimulating tumor immunity after thermal ablation. METHODS： The model of hepatoma was established with Hepa 1 - 6 cells injected into the subcutaneous tissue of C57BL/6J mice. The tumors were treated by microwave ablation under different temperatures, and then fluorescent- labeled iDCs （PKI-L26- DC） were injected into the ablated tumor tissues. The influences of ablation to homing of PKH26 - DC, maturation and excitation ability towards T - cell were observed. RESULTS： There were no homing PKH26 - DCs and expression of CCR7 in draining lymph nodes in （ 65 ± 5） ℃ and （90 ± 5） ℃ ablation groups after intratumoral injection of iDCs. The number of homing PKI-L26 - DCs was 32 ± 8 in （50 ± 5）℃ ablation group vs 21 ± 6 in unablated group, and the expression rate of CCR7 was 100% vs 90%. The number of clusters with immunological synapsis was 8 - 12 in （50 ±5）℃ ablation group vs 4- 6 in un- ablated group under 100- magnification visual field, and the number of lymphocytes in each cluster was 12 - 25 vs 3 - 10. CONCLUSION： Thermal ablation of hepatoma under an appropriate temperature level may promote maturing and homing of iDCs and stimulate immunity of lymphocytes.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第2期355-359,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30471686)
关键词
肝肿瘤
消融
树突细胞
归巢
Liver neoplasms
Ablation
Dendritic cells
Homing
