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山莨菪碱对乙醇诱导脑损伤大鼠海马胶质纤维酸性蛋白表达的影响

Effect of anisodamine on the expression of glial fibriliary acidic protein in hippocampus of rats with the ethanol-induced brain damage
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摘要 目的:探讨山莨菪碱对乙醇诱导大鼠脑损伤模型大鼠学习记忆障碍的改善作用及对海马结构的神经胶质细胞内胶质纤维酸性蛋白变化的保护作用。方法:实验于2004-10/2005-06在解放军总医院老年医学研究所病理生理室完成。选择约2月龄SD雄性大鼠72只,随机分成生理盐水组、乙醇组、山莨菪碱+生理盐水组、山莨菪碱+乙醇组,每组18只。以无水乙醇溶液腹腔注射(13mL/kg,1次/d,连续8d)诱导大鼠脑损伤。山莨菪碱+生理盐水组、山莨菪碱+乙醇组腹腔注射山莨菪碱3mL/kg,1次/d,连续8d。生理盐水组腹腔注射生理盐水13mL/kg,1次/d,连续8d。分别在大鼠给药的第1,3,5,7天称量体质量,观察4组大鼠的体质量变化。采用Morris水迷宫测试大鼠1min内在4个象限找到平台的时间、流动路程和速度等各项指标。采用免疫组化技术并结合图像分析系统,测定海马CA1,CA3,DG区胶质纤维酸性蛋白表达阳性的胶质细胞的计数和平均面积百分比,以定量分析乙醇对海马及齿状回结构和功能的影响及山莨菪碱的作用。结果:实验过程中生理盐水组1只大鼠和乙醇组2只大鼠死亡、山莨菪碱+生理盐水组、山莨菪碱+乙醇组无脱失。4组大鼠进入结果分析数量分别为17、16、18、18只。①乙醇组在Morris水迷宫中寻找平台的潜伏期时间、流动路程明显长于较生理盐水组、山莨菪碱+生理盐水组、山莨菪碱+乙醇组,差异有显著性[(26.14±21.68)s,(14.48±13.63)s,(16.22±14.89)s,(18.49±16.21)s,P<0.05];[(367.19±334.31)cm,(229.87±236.13)cm,(260.22±269.68)cm,(298.52±272.88)cm,P<0.05]。②胶质纤维酸性蛋白表达阳性的神经胶质细胞计数:乙醇组在海马CA1、CA3区均高于生理盐水组、山莨菪碱+生理盐水组、山莨菪碱+乙醇组,差异有显著性[(22.83±2.41),(18.39±3.38),(19.39±2.91),(19.03±3.19),P<0.05];[(25.97±2.50),(22.17±3.31),(21.67±2.33),(23.40±2.85),P<0.05];③胶质纤维酸性蛋白 AIM: To investigate the effects of anisodamine in ameliorating the learning and memory disorders of rats with ethanol-induced brain damage, and its protective effect on the changes of glial fibriliary acidic protein (GFAP) in hippocampal structural neuroglial cells. METHODS: The experiment was conducted in the Laboratory of Pathophysiology in the Institute of Geriatrics and Gerontology, General Hospital of Chinese PLA from October 2004 to June 2005. Seventy-two heahy male Sprague-Dwley rats of about 2 months old were randomly divided into 4 groups with 18 rats in each: saline group, ethanol group, anisodamine+saline group and anisodamine+ethanol group. Brain injury was induced by intraperitoneal injection of anhydrous ethanol (13 mL/kg, once a day for 8 continuous days). Rats in the anisodamine+saline group and anisodamine+ethanol group were treated with intraperitoneal injection of anisodamine 3 mL/kg, once a day for 8 continuous days. Rats in the saline group were given intraperitoneal injection of saline 13 mL/kg, once a day for 8 continuous days. The body mass of the rats were measured at 1, 3, 5 and 7 days after administration respectively, and the changes of body mass were observed in the 4 groups. The time, distance and rate for the rats to find the platform within 1 minute were checked with the Morris water maze test. The counts and mean area of GFAP positve cells in rats' hippocampal CA1, CA3 and DG areas in the 4 groups were measured by immunohistochemical technique and image analytical system, so as to quantitatively analyze the influence of ethanol on the structure and function of hippocampus and dentate gyrus, and the role of anisodamine. RESULTS: During the experiment, 1 and 2 rats died in the saline group and ethanol group respectively, and there was no deletion in the anisodamine+saline group and anisodamine+ethanol group, finally 17, 16, 18 and 18 rats entered the analysis of results in the 4 groups respectively. ① The latency and distance for the rats to find the
出处 《中国临床康复》 CSCD 北大核心 2006年第2期123-125,共3页 Chinese Journal of Clinical Rehabilitation
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