摘要
目的呼吸道上皮细胞在固有免疫和炎症反应中都扮演重要角色。本文旨在研究强效吸入型糖皮质激素丙酸氟替卡松(FP)对双链RNA(dsRNA,Toll样受体3的配体)刺激的呼吸道粘膜上皮细胞BEAS-2B产生的某些固有免疫相关蛋白和炎性细胞因子的作用。方法FP(10-7mol.L-1)作用于BEAS-2B 2 h后,加入dsRNA 25 mg.L-1继续培养18 h,提取细胞RNA和收集细胞培养上清液。实时定量PCR测定炎性细胞因子IL-8、GM-CSF、IL-1β和固有免疫蛋白血清淀粉样蛋白(SAA)、补体B因子、分泌性白细胞蛋白酶抑制物(SLPI)的mRNA含量;ELISA分析上清液中IL-8、GM-CSF、SAA蛋白水平。结果BEAS-2B细胞在dsRNA刺激后,IL-8、GM-CSF、IL-1β、SAA、补体B因子、SLPI的mRNA均增高。FP预处理的细胞,在dsRNA刺激后,SAA、补体B因子、SLPI的mRNA水平不降低,IL-8、GM-CSF、IL-1β的mRNA水平明显降低。IL-8、GM-CSF、SAA的ELISA分析证实了mRNA的试验结果。结论FP在强效抗炎的同时,并不损伤呼吸道上皮局部的固有免疫。
Aim Airway epithelial cells play importam roles in innate immunity and inflammatory responses in the airways. We investigated the effect of the potent inhaled glucocorticoid FP on expressions of both innate immune effectors and inflammatory mediators in BEAS- 2B airway epithelial cells after stimulation with Tolllike receptor 3 ligand double stranded RNA （dsRNA）. Methods BEAS-2B airway ted with FP （ 10^-7 mol · L^-1 ）for 2h before stimulation with dsRNA （25 mg ·L^-1）. RNA was isolated and the cell supernant was collected, mRNA for the inflammatory cytokines IL-8, GM-CSF, IL-1β and the innate immunity effectors serum amyloid A （SAA）, complement B factor, secretory leukocyte protease inhibitor （SLPI） were quantified by real-time RT-PCR.The protein level of IL-8, GM-CSF and SAA in the su- pernant was analyzed by ELISA. Results Stimulation of BEAS-2B cells with dsRNA increased the mRNA levels of IL-8, GM-CSF, IL-Iβ, SAA, B factor and SLPI. Treatment with FP did not inhibit induction of mRNA for SAA, B factor and SLPI. FP did inhibit ex- pressions of mRNA for IL-8, GM-CSF, IL-1β. ELISA of IL-8, GM-CSF and SAA confirmed the mRNA expression results. Conclusion Our results suggest that FP may spare innate responses in airway epithelial cells while it inhibit expression of inflammatory mediators.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第1期67-70,共4页
Chinese Pharmacological Bulletin
基金
美国国立卫生院(NIH)资助项目(NoHL48544
AI44885
AI50530)
安徽省优秀青年科技基金资助项目(No04043053)
