摘要
目的体外评价Β-半乳糖苷酶基因工程乳酸菌缓解乳糖对细胞毒性作用的效果。方法建立乳糖细胞毒性作用CACO-2细胞模型,采用形态学观察及细胞增殖活性等指标检测该菌对乳糖细胞毒性的作用效果。结果成功建立乳糖细胞毒性作用CACO-2体外模型,所构建的Β-半乳糖苷酶基因工程乳酸菌能够保护细胞耐受乳糖毒性而呈现正常形态,并能提高乳糖作用下的细胞活性(P<0.01)。结论Β-半乳糖苷酶基因工程乳酸菌在体外可显著的降低乳糖的细胞毒性作用,为进行其食品级改造奠定了基础。
Objective To assess the relief effect of β-galaetosidase genetically engineered Lactococcus lactis on the cell toxicity caused by lactose in vitro. Methods An in vitro toxic Caeo-2 cell model caused by lactose was established to evaluate the relief effect of β-galaetosidase genetically engineered Lactococcus lactis. Cell morphological parameters and proliferation activity parameter were used. Results The in vitro toxic Caeo-2 cell model caused by lactose was successfully established; the genetically engineered Lactococcus lactis constructed in the authors＇ laboratory could enable the Caeo-2 cell to have normal appearance with the presence of lactose and could improve the proliferation activity with the presence of high concentration of lactose （P〈0. 01）. Conclusion The β-galaetosidase genetically engineered Lactococcus lactis has significant relief effect on the cell toxicity caused by lactose in vitro, which lays a foundation for food-grade alternation of this bacterium.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第1期52-54,共3页
Journal of Sichuan University(Medical Sciences)
基金
四川省卫生厅基金(F0201)资助
