摘要
目的探讨化疗后结直肠癌组织Fas/FasL和survivin的表达情况及两者之间的相关性。方法采用脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记技术(TUNEL法)和免疫组化方法,测定化疗后和未化疗两组各16例结直肠癌患者肿瘤的原位凋亡(AI)、原位增殖(KI)及survivin表达,运用流式细胞仪测定肿瘤细胞的细胞周期、Fas/FasL表达,用双抗体ELISA法检测患者血清可溶性Fas/FasL(sFas/sFasL)表达。结果化疗组结直肠癌患者肿瘤细胞的AI及Fas/FasL均较对照组高,KI、G2/M期细胞、sFas及survivin较对照组低,差别有统计学意义(P<0.05)。结论化疗通过诱导结直肠癌细胞凋亡及阻止细胞进入G2/M期从而抑制癌细胞的生长。Fas/FasL系统与结直肠癌细胞凋亡、增殖调控有关。survivin阳性表达下降程度可作为化疗有效的标志之一。
Objective： To investigate the expressions of Fas/FasI, and survivin in colorectaI cancer cells after chemotherapy and the correlationship between them. Methods： Thirty-two colorectraI cancer patients were divided into two groups randomly. One group （ n= 16） received chemotherapy and the control group（ n =16） did not. The apoptotie cells index （AI） in situ was identified by terminal deoxynucleotidyI transferase biotin-dUTP nick end labeling （TUNEL） assay. ImmunohistochemicaI method was used for measurement of Ki67 proliferation index （KI） and survivin expression. Ceil cycle and Fas/FasL expression were assessed by Flow Cytometry. The serum soluble Fas/FasL （sFas/FasL） was determined by enzyme linked immunosorbent assay （ELISA）. Results： The AI and Fas/FasL expression were higher in the chemotherapy group than in the control group. The KI, G2/M cycle arrest, sFas and survivin expression were lower in the chemotherapy group than in the control group. The difference was significant（ P〈0.05）. Conclusions： Preoperative chemotherapy can effectively inhibit colorectal cancer cells proliferation by inducing the apoptosis and G2/M arrest of the cancer cells at G2/M stage. Fas/Fasg system is related with apoptosis and proliferation of colorectaI cancer cells. Survivin expression provides prognostic information that may have important therapeutic implications.
出处
《肿瘤》
CAS
CSCD
北大核心
2006年第1期85-87,共3页
Tumor
