摘要
目的:研究小核核糖核蛋白多肽N基因(SNRPN)在肝癌肿瘤HepG2细胞株的表达及基因印迹状态.方法:采用RT-PCR方法检测出SNRPN基因在肝癌肿瘤HepG2细胞株中的表达状况,对HepG2细胞株基因组DNA和cDNA中的SNRPN基因外显子4nt1654312位点用RT-PCR为基础的RFLP方法进行基因分型.结果:HepG2细胞稳定表达SNRPN,SNRPN外显子4nt1654312(数字依据NT_026446,SNPrs705)为杂合子(C/T);RT-PCR为基础的RFLP分析表明,SNRPN的双等位基因中只有T等位基因产生mRNA转录本.结论:SNRPN基因在HepG2肝癌细胞株中有表达,其基因印迹状态未丢失.
AIM： To investigate the expression and imprinting style of small nuclear ribonucleoprotein polypeptide N （SNRPN） in hepatic cancer cell line HepG2.
METHODS： Human hepatic cancer cell line HepG2 was cultured in vitro by routine method. The expression of SNRPN gene in the cells was detected by reverse transcription polymerase chain reaction （RT-PCR）. The single nucleotide polymorphisms （SNP） of SNRPN at exon 4 nt 1654312 （numbered according to NT_026446, SNP rs705） was genotyped in a genomic DNA sample and a cDNA sample of HepG2 cell line with restriction fragment length polymorphism （RFLP） based on RT-PCR.
RESULTS： SNRPN was stably expressed in HepG2 cells. The heterozygote C/T was found at exon 4 nt 1654312 of SNRPN. In cell lines heterozygous with respect to this SNP, only one of the two alleles （T allele） present in the genomic DNA produced an mRNA transcript.
CONCLUSION： SNRPN mRNA is expressed in HepG2 cells, and there is no loss of imprinting.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第21期2545-2548,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30470964~~
关键词
小核核糖核蛋白多肽N
基因印迹
限制性片段长度
多态性
杂合子
Small nuclear ribonucleoprotein polypeptide N
Genomic imprinting
Restriction fragment length polymorphism
Heterozygote
