摘要
红细胞输注是输血医学的主体,化学材料甲氧基聚乙二醇(mPEG)与红细胞膜共价结合后,可遮蔽红细胞表面血型抗原,使红细胞血型抗原呈阴性,即形成通用型红细胞,对临床输血具有重要意义。研究了mPEG修饰对红细胞血型抗原、膜流动性的影响;红细胞被修饰程度;mPEG与红细胞结合的稳定性;mPEG在体内的代谢情况。结果表明,mPEG能遮蔽红细胞上的血型抗原;mPEG与红细胞结合稳定;mPEG修饰红细胞的比率随浓度增高而增大;mPEG在小鼠体内24 h后基本被代谢,且在体内无蓄积。mPEG修饰的红细胞为解决临床急用稀有血型和配型困难(如自身溶血性贫血,AIHA)等问题提供了有意义的参考。
Transfusing red blood cell is a crucial component of modem blood transfusion. Previous studies have showen that methoxypolyethylene glycol （mPEG） can mask RBC surface antigens and attenuate RBC antigenicity, and thus agglutination with antibodies is decreased. The aim of this study was to detect the effect of mPEG modification on RBC antigens and membrane fluidity, to investigate the ratio of modified RBC, to determine possible sites of covalent mPEG derivatization, to assay the combined stability of mPEG on red cell membrane and to detect the toxcitity of mPEG in vivo. The results showed that the ratio of modified RBC was increased in a dose-dependent manner by twophase partitioning system; mPEG could not detach from RBC after pegylation even RBC was destroyed, while remaining antigenically silent; mPEG modification did not affect membrane fluidity; almost all exterior-protein on membrane were modified by mPEG; and mPEG was metabolized in vivo safely and no cumulation in recipient mouse. In conclusion, mPEG-RBCs have acceptable in vitro properties and it provides a useful strategy for resolving problem of clinical blood matching, though such masking still have specific problems to be resolved.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2006年第1期1-5,共5页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金重点项目(9732002CB713804)
关键词
甲氧基聚乙二醇
红细胞
血型抗原
输血
methoxypoly（ethylene glycol）
red blood cell
blood group antigen
transfusion
